| 高效液相法测定脑栓康复浓缩丸葛根中葛根素的含量 |
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| 引用本文： | 刘宏,王兰英,任建国,赵立军. 高效液相法测定脑栓康复浓缩丸葛根中葛根素的含量[J]. 北方药学, 2013, 0(1): 6-7 |
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| 作者姓名： | 刘宏 王兰英 任建国 赵立军 |
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| 作者单位： | 内蒙古医科大学药学院;内蒙古奥特奇蒙药股份有限公司 |
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| 摘 要： | 目的：建立脑栓康复浓缩丸葛根中葛根素的含量测定方法。方法：采用反相高效液相色谱法（RP-HPLC）,色谱柱DiamosilC18柱（4.6mm×250mm,5μm）,流动相为甲醇-0.1%磷酸溶液（22：78）;检测波长为250nm,流速为1ml/min,进样量为10μl。结果：葛根素的线性范围为0.202～3.232μg（r=0.9998）,平均加样回收率为（N=6）100.30%,RSD为1.35%。结论：本法准确、简便、重复性好,可作为脑栓康复浓缩丸的质量控制方法。
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| 关 键 词： | 高效液相色谱法 葛根素 脑栓康复浓缩丸 含量测定 |
Determination of Puerarin in Naoshuankangfu concentrated pills by HPLC |
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| Liu Hong,Wang Lanying,Ren Jianguo,Zhao Lijun. Determination of Puerarin in Naoshuankangfu concentrated pills by HPLC[J]. BEIFANG YAOXUE, 2013, 0(1): 6-7 |
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| Authors: | Liu Hong Wang Lanying Ren Jianguo Zhao Lijun |
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| Affiliation: | 1.Pharmacology School,Inner Mongolian Medical University,Inner Mongolia 010110;2.Inner Mongolia Otaqi Mongolia Medicine Co.,Ltd.010110) |
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| Abstract: | Objective：To establish a HPLC method for determining the content of Puerarin in Naoshuankangfu concentrated pills. Methods：A chn）matographic column of Diamosil C ls（4.6mm×250mm, 5μm）was used, the mobile phase was methanol-0.1%Phosphoric acid （22：78）.The flow velocity was lml/min,detection wave length was 250nm.Results：The linear range of Puerarin was 0.202 - 3.232μg （r=0.9998）,average recovery （n=6）100.30%（RSD1.35%）.Conclusions：The method Was accurate and simple with a higher repeatability, which Can be used for the quality control of Naoshuankangfu concentrated pills. |
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| Keywords: | HPLC Puerarin Naoshuankangfu concentrated pills Quantitative determination |
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