当归注射液治疗再生障碍性贫血的实验研究

目的 :探讨当归注射液对再生障碍性贫血 (AA)小鼠骨髓造血功能的保护作用。方法 :雌性Balb/c小鼠 ,随机分为 5组。①正常对照组 ,未经特殊处理。②模型对照组 ,经60 Co 6 .0Gyγ 射线亚致死量全身照射 ,4d内由尾静脉输入取自DBA/ 2小鼠的胸腺、淋巴结混合细胞 ,制成AA模型 ,于制模当天给予腹腔注射无菌生理盐水 ,10ml·kg-1·d-1,连续 12d。③当归注射液小剂量组 ,于制模当天即给予当归注射液腹腔注射 2ml·kg-1·d-1,连续 12d。④当归注射液大剂量组 ,予当归注射液 10ml·kg-1·d-1,腹腔注射。⑤丙酸睾丸酮组 ,给予腹腔注射丙酸睾丸酮 10ml·kg-1·d-1。于第 12d每只小鼠称重后 ,采集尾静脉血测外周血白细胞计数 ,后断颈处死小鼠 ,取出双侧尺骨及股骨 ,冲出骨髓单个核细胞 ,计数。并行CD34+ 细胞检测及骨髓超微结构观察。结果 :与正常对照组比较 ,模型对照组造血细胞明显减少 ,骨髓微环境受损。当归注射液大剂量组能明显改善骨髓增生程度 ,修复骨髓微环境。结论 :当归注射液能通过促进骨髓造血细胞增生 ,改善骨髓微环境对AA小鼠进行保护作用。

A Study of Angelica Sinenisis Injection on Aplastic Anemia Mice

Objective: To investigate the protective effect of angelica sinenisis injection on hematopoiesis of aplastic anemia(AA) mice. Methods: The female Balb/c mice were divided into 5 groups. Group A was normal control. Group B had been generally irradiated by sub-lethal dosage 60 Co 6 Gy γ-ray, then injected through tail vein with mixed lymphocytes of thymus gland and lymphoid node of DBA mice in 4 hours. After AA models were established, each mouse was intraperitoneally injected with sterile saline 10 ml·kg -1 ·d -1 for 12 days. Mice from Group C were intraperitoneally injected with angelica sinenisis injection 2 ml·kg -1 ·d -1 for 12 days for an intervention treatment. Mice from Group D were intraperitoneally injected with angelica sinenisis injection 10 ml·kg -1 ·d -1 for 12 days. Mice from Group E were intraperitoneally injected with testosterone propionate 10 ml·kg -1 ·d -1 . All mice were killed by cutting neck on the 12th day, and ulnas and femur were taken out. The bone marrow ultrastructure of the mice ulnas were observed. The number of femnr bone marrow mononucleate cells(BMNC)were measured and the CD34 expression levels were measured by the flow cytometor analysis system. Results: The expression levels of CD34 in Group B were lower than those in Group A (P<0.01), but they were increased in Group D (P< 0.01 ). The ultrastructure observation showed that the mitochondria and endoplasmic reticulum of hematopoietic cells in Group B had been reduced and aberrant, and the blood sinus cavity was expanded and the wall of sinus was broken. In Group D, the mitochondria and endoplasmic reticulum were plentiful and normal in shape, and the blood sinus were almost normal. No obvious difference was found between Group D and Group E in MNC counting and hematopoietic microenvironment. Conclusion: Angelica sinenisis injection plays a protective role in aplasitic anemia mice .Because it could promote the proliferation of bone marrow of mice, it could improve the hematopoietic microenvironment.