缺铁性贫血患儿T辅助细胞的免疫与调控

为研究缺铁性贫血患儿T辅助细胞 (TH)的细胞调控及免疫功能。对 2 0 0 2年 1月至 2 0 0 2年 1 2月住院患儿进行TH 细胞检测 ,同时设正常健康儿童 2 8人为对照组。对两组儿童应用ELISA法检测白细胞介素 2 (IL 2 )及γ干扰素 (INF γ)水平 ,用单细胞内染色法检测TH1、TH2 百分率。结果 :缺铁贫血患儿IL 2 ( 1 6 55± 2 87ug L)水平明显降低 ,与对照组IL 2 ( 2 4 73± 4 37ug L)比较差异有显著意义 (t =8 6 2 ,P <0 0 1 ) ;INF γ( 4 75 6± 2 7 1pg mL)水平也明显降低 ,与对照组IFNγ( 6 54 0 7± 1 4 6 4pg mL)比较 ,差异有极其显著性意义 (t =7 4 2 ,P <0 0 1 ) ;缺铁性贫血患儿TH1百分率( 1 2 2 4± 2 51 % )亦明显低于对照组TH1百分率 ( 1 6 6 7± 2 73% ) ,经t检验差异亦有极显著意义 (t=6 89,P <0 0 1 ) ;缺铁性贫血患儿TH2 百分率明显升高 (t=5 37,P <0 0 5)。结果表明 :缺铁贫血患儿机体TH1细胞数量及功能低下 ,TH1 TH2 值下降 ,TH1、TH2 极化异常 ,导致细胞毒性T细胞 (CTL)介导的细胞免疫功能受到抑制

Immune Function and Immune Regulation of TH in Children with Iron Deficiency Anemia

To explore the immune regulation and immune function of T H in children with iron deficiency anemia. Methods IL-2, IFN-γ were tested by ELISA while T H1, T H2 by colouring within single cell. Above indexes were tested in 38 cases of children with Iron deficiency anemia before treatment, 28 normal children were investigated as controls, Result IL-2, IFN-γ, T H1 in patients were significantly decreased compared with normal controls (P<0.01). T H2 were significantly increased in patients compared with normal controls (P<0.05). Conclusions: The quantity of T H1 cells were decreased and immune function reduced in children with iron deficiency anemia.