| HBeAg阳性慢性乙型肝炎孕妇胎盘组织Toll样受体3的表达及意义 |
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| 引用本文： | 资捷,王前,郑磊,熊石龙,蔡贞. HBeAg阳性慢性乙型肝炎孕妇胎盘组织Toll样受体3的表达及意义[J]. 中国实用妇科与产科杂志, 2010, 0(4) |
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| 作者姓名： | 资捷 王前 郑磊 熊石龙 蔡贞 |
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| 作者单位： | 南方医科大学南方医院检验医学中心;深圳市福田妇幼保健院检验科; |
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| 摘 要： | 目的探讨孕妇胎盘组织Toll样受体3(TLR3)的表达与乙型肝炎病毒(HBV)持续感染的关系。方法2008年11月至2009年5月在南方医科大学南方医院产科选取22例HBeAg阳性(HbeAg阳性组)和24例HBeAg阴性(HBeAg阴性组)慢性乙型肝炎(乙肝)孕妇,及正常孕妇25例。建立实时荧光定量RT-PCR检测胎盘组织中TLR3mRNA水平,同时以免疫组化法检测其在胎盘组织中的表达,并采用实时荧光定量PCR和微粒子酶免疫分析法(MEIA)分别定量检测其血清HBV-DNA和HBeAg水平。结果HBeAg阳性孕妇胎盘组织中TLR3mRNA水平(×10-1)(3.204±0.395)明显低于正常对照组(7.834±0.970)和HBeAg阴性组(5.746±0.580),两组相比P0.01;HBeAg阳性孕妇血清HBeAg及HBV-DNA水平[562.2±337.4和(7.15±3.69)×107copy/mL]明显高于正常对照组(0.29±0.06,1.00×103copy/mL)和HBeAg阴性组[0.35±0.07,(2.40±1.71)×103copy/mL](P0.01);胎盘组织中TLR3mRNA表达与HBeAg水平呈负相关(r=-0.495,P0.05),而与血清HBV-DNA水平无显著相关性。结论胎盘组织中TLR3的表达与其持续感染HBV有一定关系,HBeAg对胎盘组织TLR3表达起负调节作用。
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| 关 键 词： | Toll样受体3 乙型肝炎病毒 乙型肝炎病毒e抗原 胎盘 荧光定量RT-PCR |
TLR3 expression in placenta of pregnant women with HBeAg-positive chronic hepatitis B |
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| ZI Jie,WANG Qian,ZHENG Lei,XIONG Shi-long,CAI Zhen. Laboratory Medicine Center,Nanfang Hospital,Southern Medical University,Guangzhou ,China. TLR3 expression in placenta of pregnant women with HBeAg-positive chronic hepatitis B[J]. Chinese Journal of Practical Gynecology and Obstetrics, 2010, 0(4) |
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| Authors: | ZI Jie WANG Qian ZHENG Lei XIONG Shi-long CAI Zhen. Laboratory Medicine Center Nanfang Hospital Southern Medical University Guangzhou China |
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| Affiliation: | ZI Jie,WANG Qian,ZHENG Lei,XIONG Shi-long,CAI Zhen. Laboratory Medicine Center,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China |
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| Abstract: | Objective To explore the relationship of TLR3 expression and HBV persistent infection in placenta of pregnant women.Methods To choose 22 HBeAg-positive and 24 HBeAg-negative chronic hepatitis B(CHB)pregnant women,and 25 normal pregnant women in our hospital obstetrics between November 2008 and May 2009.To detect the expression of TLR3 mRNA in placentas by real-time fluorescence quantitative RT-PCR(qRT-PCR)and immunohistochemical method.To assay the level of HBV DNA and HBeAg by real-time fluorescence quanti... |
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| Keywords: | Toll-like receptor 3 hepatitis B virus HBeAg placenta qRT-PCR |
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