新生儿缺氧缺血性脑病NO、GSH—PX和TNF的含量测定及其临床意义

目的探讨新生儿缺氧缺血性脑病(HIE)血浆和脑脊液(CSF)中的一氧化氮(NO)和谷胱甘肽过氧化物酶(GSH-PX)和肿瘤坏死因子(TNF)含量变化及其与HIE不同时期和不同程度间的相关关系.方法对HIE患儿第3天的CSF和出生初入院(HIE2h内)、第1天、第2天、恢复期的血浆中NO、SOD进行检测,并与正常对照组比较;分析NO、GSH-PX、TNF的变化原因和意义.结果HIE患儿血浆中第1天的NO含量最高,而GSH-PX相反;初入院、第1天、第3天的血浆中NO、GSH-PX含量与正常对照组对比均有显著性差异(P＜0.01),而恢复期中NO、GSH-PX含量与正常对照组对比无显著性差异(P＞0.05),血浆和HIE第3天的CSF中NO和GSH-PX水平均呈负相关;病情越重NO浓度越高,GSH-PX越低.急性期血浆TNF显著高于对照组(P＜0.01),恢复期两组无显著性差异(P＞0.05).结论NO、GSH-PX和TNF参与HIE的发病过程,在HIE的发病过程中起着重要作用;检测血浆和CSF中NO、GSH-PX、TNF含量有助于判断HIE患儿病变程度和病情进展.

Testing the concentrations of NO, GSH-PX and TNF of newborn infants with HIE

Objection To study the expression of the nitric oxide(NO),Glutathion Peroxidase(GSH-PX)and Tumor necrosis factor(TNF)in plasma and cerebrospinal fluid(SCF)of neonate hypoxic-ischemic enceph alopathy(HIE)and the relations among their expressions in different periods and degrees of HIE.Methods Tested the concentrations of NO and GSH-PX and TNF in CSF of newborn infants suffered HIE 3 days and in plasma of newborn infants suffered HIE just attacked instant(HIE within 2 hours),1 day,3 days and restoring stage and Compared with them of normal contrast term.Analysed the reasons and significance of NO,GSH-PX and TNF changes Results The NO concentration is the highest in plasma of newborn infants suffered HIE 1 day.The GSH-PX concentration is the opposite .There are founded to have significant differences( P <0 01) after comparing the NO concentrations and the GSH-PX concentrations in plasmas of newborn infants suffered HIE just attacked instant ,1 day,3 days with of normal contrast team respectively.But there isn't significant difference( P >0 05)between them in plasmas of HIE on restoring stage and of normal contrast team.It is negative correlation whatever between the NO concentration and the GSH-PX concentration in plasma or in CSF of newborn infants suffered HIE 3 days.The more serious the disease is,the higher the NO concentration is,the lower the GSH-PX concentrations is. There is significant difference between the TNF concentrations in plasma of HIE and normal contrast team during acute periods,but there isn't difference in restoring period.Conclusion NO, GSH-PX and TNF play along with the course of HIE and play a important role in neonate HIE.Testing the concentration of NO, TNF and GSH-PX in plasma and in CSF can also help to judge the degree of disease and the course of disease.