| HPLC法同时测定大枣中芦丁和酸枣仁皂苷B的含量 |
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| 引用本文： | 陈鑫,刘永刚,王耀欣,郑丽英,何希辉,韩南银.HPLC法同时测定大枣中芦丁和酸枣仁皂苷B的含量[J].中国药房,2010(3):247-248. |
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| 作者姓名： | 陈鑫 刘永刚 王耀欣 郑丽英 何希辉 韩南银 |
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| 作者单位： | [1]北京大学医学部药学院,北京市100191 [2]北京中医药大学中药学院,北京市100102 |
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| 摘 要： | 建立以高效液相色谱法同时测定大枣中芦丁和酸枣仁皂苷B含量的方法。方法:色谱柱为Agilent Extent-C1(8250mm×4.6mm,5μm),流动相为0.01%磷酸水溶液-乙腈(梯度洗脱),流速为1mL·min-1,变波长检测。结果:芦丁和酸枣仁皂苷B的进样量分别在1.38～6.88μg(r=0.9998)和0.448～2.24μg(r=0.9999)范围内与各自峰面积积分值呈良好线性关系;平均回收率分别为99.14%(RSD=1.13%,n=6)和99.94%(RSD=2.22%,n=6)。结论:本方法准确、稳定、可靠,可用于大枣的质量控制和评价。
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| 关 键 词： | 大枣 芦丁 酸枣仁皂苷B 高效液相色谱法 含量测定 |
Simultaneous Determination of Rutin and Jujuboside B in Ziziphusjujuba by HPLC |
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| CHEN Xin,WANG Yao-xin,ZHENG Li-ying,HE Xi-hui,HAN Nan-yin.Simultaneous Determination of Rutin and Jujuboside B in Ziziphusjujuba by HPLC[J].China Pharmacy,2010(3):247-248. |
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| Authors: | CHEN Xin WANG Yao-xin ZHENG Li-ying HE Xi-hui HAN Nan-yin |
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| Institution: | , LIU Yong-gang(School of Pharmacy, Peking University Health Science Center, Beijing 100191, China; College of Traditional Chinese Medicine, Beijing University of TCM, Beijing 100102, China) |
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| Abstract: | OBJECTIVE：To establish a HPLC method for simultaneous determination of rutin and jujuboside B in Ziziphus jujuba. METHODS： The separation was performed on an Agilent Extent-C18（250mm×4.6mm,5μm） column. The mobile phase was 0.01% phosphoric acid aqueous solution and acetonitrile （gradient elution） with flow rate of 1 mL·min^-1 and variable wavelength. RESULTS： The linear ranges of rutin and Jujuboside B were 1.38-6.88 μg（r=0.999 8） and 0.448-2.24 μg （r=0.999 9）, respectively. The average recoveries were 99.14% （RSD=1.13% ,n=6） and 99.94% （RSD=2.22% ,n=6）,respectively. CONCLUSION： This method is aceurate, stable and reliable for quality control and determination of Z. juiuba. |
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| Keywords: | Ziziphusjujuba Rutin Jujuboside B HPLC Content determination |
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