人参与藜芦配伍后人参皂苷类成分煎出量变化研究

目的:研究人参与藜芦配伍后人参皂苷类成分煎出量的变化,找出2味药配伍后在化学成分上的变化规律,阐释中药配伍理论中"人参反藜芦"的机制。方法:通过高效液相色谱(HPLC)法测定人参与不同剂量藜芦配伍后水煎液中人参皂苷的含量;采用比色法测定药渣中人参总皂苷的含量。结果:人参与藜芦配伍后人参皂苷的含量降低,并且随着藜芦配伍剂量的增加人参皂苷逐渐减少;但人参与藜芦水煎后的残渣中人参皂苷含量并没有因为藜芦加入量增加而增加。结论:"人参反藜芦"有一定科学道理。随着藜芦加入量的增加人参皂苷减少并不是因为藜芦的某些成分抑制人参皂苷在水溶液中的溶出,而可能是藜芦中某些成分与人参皂苷发生了化学反应导致人参皂苷的减少。

Study on the Change of the Content of Ginsenosides in the Decoction of Panax ginseng and Veratrum nigrum

OBJECTIVE：To study the change of the content of ginsenosides in the decoction of Panax ginseng and Veratrum nigrum and to find out the change regularity of chemical components in the compatibility of P. ginseng and V. nigrum so as to introduce the mechanism of ＂P. ginseng and V. nigrum are incompatible with each other＂ which derives from compatibility theory of Traditional Chinese Medicine. METHODS：HPLC was adopted to determine the content of ginsenoside in decoction derived from the combination of P. ginseng with different amounts of V. nigrum,and colorimetry was applied to determine the content of the total ginsenosides in herbal esidues. RESULTS：The content of the total ginsenosides decreased in the decoction as long as the content of V. nigrum increased. Nevertheless,the content of ginsenosides didn＇t change as amount of V. nigrum increased. CONCLUSION：It is reasonable to some extent that ginseng and veratrum nigrum are incompatible with each other. With the amount of V. nigrum increasing,the decrease of the total ginsenosides content doesn＇t result from some components in V. nigrum inhibiting the dissolution of ginsenoside in water solution but result from some components in V. nigrum reacting with ginsenoside leading to the reduction of ginsenoside.