川产迭鞘石斛中香豆素的分离鉴定及药材质量标准研究

目的:建立川产迭鞘石斛药材的质量标准。方法:对川产迭鞘石斛药材进行香豆素对照品的提取分离,并建立其薄层色谱(TLC)定性鉴别及高效液相色谱含量测定方法。结果:从药材中分离并鉴定出香豆素标准品。TLC鉴别斑点清晰;香豆素进样量在0.096～0.480μg范围内与峰面积积分值呈良好线性关系,平均回收率为99.1%,RSD=1.44%(n=6)。结论:本试验结果可较全面反应川产迭鞘石斛的内在质量,并为有效评价迭鞘石斛药材的质量和开发川产迭鞘石斛资源提供依据。

Isolation and Identification of Coumarin in Dendrobium denneanum Cultivated in Sichuan and Its Quality Standard Study

OBJECTIVE：To establish the quality creteria of Dendrobium denneanum cultivated in Sichuan. METHODS： The reference substance had been isolated and identified. HPLC and TLC were applied for determination and identification respectively, RESULTS： The coumarin control had been successfully isolated and the TLC spots were clear. The linear range of coumarin was 0.096--0.480 ~tg with an average recovery of 99.1% （RSD=1.44%, n=6）. CONCLUSION： The results can overall reflect the internal quality of D. denneanum cultivated in Sichuan and provide basis for quality evaluation and development of D. denneanum from Sichuan.