| RP-HPLC法测定施普睿达原料药中主药的含量 |
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| 引用本文： | 徐阳,刘培勋,禹洁,王育苗,龙伟,马世堂. RP-HPLC法测定施普睿达原料药中主药的含量[J]. 中国药房, 2010, 0(13): 1231-1232 |
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| 作者姓名： | 徐阳 刘培勋 禹洁 王育苗 龙伟 马世堂 |
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| 作者单位： | 中国医学科学院北京协和医学院,放射医学研究所,天津分子核医学重点实验室,天津市300192 |
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| 基金项目： | 天津市科技计划项目（072CKFSH00200） |
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| 摘 要： | 目的:建立反相高效液相色谱法(RP-HPLC)测定施普睿达原料药中主药含量的方法。方法:色谱柱为SunFireTMRPC18,流动相为磷酸盐缓冲溶液-乙腈(9∶1,V/V),流速为1mL·min-1,检测波长为220nm,进样量为10μL,柱温为25℃。结果:施普睿达检测浓度线性范围为0.004～0.5mg·mL-1(R2=0.9999),平均加样回收率为100.0%(n=9),RSD=0.23%(n=6)。结论:该方法系统适用性好、简便、准确可靠、重复性好,适合施普睿达的含量测定。
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| 关 键 词： | 反相高效液相色谱法 施普睿达 含量测定 |
Content Determination of Main Component of Sprida Crude Drug by RP-HPLC |
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| XU Yang,LIU Pei-xun,YU Jie,WANG Yu-miao,LONG Wei,MA Shi-tang. Content Determination of Main Component of Sprida Crude Drug by RP-HPLC[J]. China Pharmacy, 2010, 0(13): 1231-1232 |
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| Authors: | XU Yang LIU Pei-xun YU Jie WANG Yu-miao LONG Wei MA Shi-tang |
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| Affiliation: | (Chinese Academy of Medical Sciences Peking Union Medical College, Institute of Radiation Medicine, Tianjin Key Laboratory of Molecular Biology and Nuclear Medicine, Tianjin 300192, China) |
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| Abstract: | OBJECTIVE： To establish an RP-HPLC method for the determination of Sprida in its crude drug. METHODS： The separation was performed on SunFireTM RP C18 column with mobile phase consisted of phosphate buffer solution-acetonitrile （9： 1, V/V） and a flow rate of 1 mL·min^-1. The detection wavelength was set at 220 nm, injection volume 10 μL and column temperature 25 ℃. RESULTS： The linear range of Sprida was 0.004-0.5 mg·mL^-1（R^2=0.999 9） with an average recovery of 100.0% （n=9） and RSD was 0.23% （n=6）. CONCLUSION： The method is feasible, simple, accurate, reliable and reproducible for the content determination of Sprida. |
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| Keywords: | RP-HPLC Sprida Content determination |
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