| HPLC法测定金顶侧耳中延胡索酸的含量 |
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| 引用本文： | 刘佳,朴惠顺,施溯筠,张善玉.HPLC法测定金顶侧耳中延胡索酸的含量[J].中国药房,2010(11):1018-1020. |
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| 作者姓名： | 刘佳 朴惠顺 施溯筠 张善玉 |
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| 作者单位： | 长白山生物资源与功能分子教育部重点实验室,延吉市133000 |
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| 摘 要： | 目的:建立以高效液相色谱法测定金顶侧耳中延胡索酸含量的方法。方法:色谱柱为大连依利特C18(250mm×4.6mm,5μm),流动相为甲醇-0.01mol·L-1磷酸二氢钾溶液(5∶95,用磷酸调pH2.5),检测波长为216nm。结果:延胡索酸进样量在0.16～1.6μg范围内与峰面积积分值呈良好的线性关系(r=0.9999);平均回收率为101.59%,RSD=1.28%(n=6)。结论:本方法简便、准确、重复性好,可用于测定金顶侧耳中延胡索酸的含量。
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| 关 键 词： | 金顶侧耳 延胡索酸 高效液相色谱法 含量测定 |
Content Determination of Fumaric Acid in Pleurotm citrinopileatus by HPLC |
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| LIU Jia,PIAO Hui-shun,SHI Su-yun,ZHANG Shan-yu.Content Determination of Fumaric Acid in Pleurotm citrinopileatus by HPLC[J].China Pharmacy,2010(11):1018-1020. |
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| Authors: | LIU Jia PIAO Hui-shun SHI Su-yun ZHANG Shan-yu |
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| Institution: | (Key Laboratory for Natural Resource of Changbai Mountain ＆ Functional Molecules, Ministry of Education, Yanji 133000, China) |
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| Abstract: | OBJECTIVE： To establish an HPLC method for the determination of fumaric acid in Pleurotm citrinopileatus. METHODS： HPLC was performed on Yilite C18 column（250 mm×4.6 mm, 5 um） with methanol-0.01 mol. L^-1 potassium dihydrogen phosphate solution（5 ： 95,pH adjusted to 2.5 using phosphoric acid） as mobile phase and detection wavelength of 216 nm. RESULTS： The linear range of fumaric acid was 0.16-1.6 ug （r=0.999 9）. The average recovery was 101.59% （RSD=l.28% ,n= 6）. CONCLUSION： This method is accurate, simple and reproducible for the content determination of fumaric acid in P. citrinopileatus. |
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| Keywords: | Pleurotm citrinopileatus Fumaric acid HPLC Content determination |
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