| HPLC法测定硫普罗宁葡萄糖注射液中硫普罗宁的含量 |
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| 引用本文： | 肖雷.HPLC法测定硫普罗宁葡萄糖注射液中硫普罗宁的含量[J].中国药房,2010(5):458-459. |
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| 作者姓名： | 肖雷 |
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| 作者单位： | 安徽医科大学第一附属医院药剂科,合肥市230022 |
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| 摘 要： | 目的:建立高效液相色谱法测定硫普罗宁葡萄糖注射液中硫普罗宁的含量。方法:色谱柱采用Diamonsil-C18,流动相为0.07mol·L-1磷酸二氢钾溶液,流速为1.0mL·min-1,检测波长为210nm。结果:硫普罗宁检测浓度在24.17～56.39μg·mL-1范围内线性关系良好(r=0.9995);平均回收率为99.06%,RSD=0.7%。结论:本方法简便、快速、准确、专属性强,适用于硫普罗宁葡萄糖注射液中硫普罗宁的含量测定。
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| 关 键 词： | 硫普罗宁葡萄糖注射液 含量测定 高效液相色谱法 |
Determination of Tiopronin in Tiopronin/Glucose Injection by HPLC |
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| XIAO Lei.Determination of Tiopronin in Tiopronin/Glucose Injection by HPLC[J].China Pharmacy,2010(5):458-459. |
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| Authors: | XIAO Lei |
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| Institution: | XIAO Lei(Dept. of Pharmacy, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China) |
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| Abstract: | OBJECTIVE： To establish an HPLC method for the determination of tiopronin in Tiopronirdglucose injection. METHODS： The separation was performed on Diamonsil-C18 column with flow rate of 1 mL·min^-1. The mobile phase composed of 0.07 mol·L^-1 NaH2PO4. The detection wavelength was 210 nm. RESULTS： Tiopronin showed good linearity within range of 24.17-56.39 μg·mL^-1（r=0.999 6）. The average recovery was 99.06% （RSD=0.7%）. CONCLUSION： The method is simple, rapid, accurate and specific for the determination of tiopronin in Tiopronin/glucose injection. |
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| Keywords: | Tiopronin/glucose injection Content determination HPLC |
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