HPLC法同时测定复方紫草颗粒中咖啡酸四聚体和丹酚酸B的含量

目的:建立以高效液相色谱法同时测定复方紫草颗粒中咖啡酸四聚体和丹酚酸B含量的方法。方法:色谱柱为YMC-PackODS-AC18(250mm×4.6mm,5μm),流动相为甲醇-0.5%磷酸水(40∶60),流速为1.0mL·min-1,检测波长为252nm,柱温为室温,进样量为10μL。结果:咖啡酸四聚体、丹酚酸B的进样量分别在0.065～1.82、0.205～5.74μg范围内与各自峰面积积分值呈良好的线性关系(r分别为0.9996、0.9997);平均回收率分别为98.72%(RSD=3.27%,n=9)、101.39%(RSD=1.81%,n=9)。结论:本方法简便、可靠、结果稳定、重复性好,可用于复方紫草颗粒的质量控制。关键词复方紫草颗粒;高效液相色谱法;咖啡酸四聚体;丹酚酸B;含量测定

Simultaneous Determination of Caffeic Acid Tetramers Cat and Salnianolic Acid B in Compound Zicao Granules by HPLC

OBJECTIVE：To develop an HPLC method for the determination of caffeic acid tetramers cat and salvianolic acid B in Compound zicao granule. METHODS：YMC-Pack ODS-A C18（250 mm×4.6 mm,5 μm） column was used with mobile phase consisted of methanol-0.5% phosphoric acid （40 ∶ 60） and flow rate set at 1.0 mL·min-1. The detection wavelength was set at 252 nm and column temperature was set at room temperature. The injection volume was 10 μL. RESULTS：The linear ranges were 0.065～1.82 μg for caffeic acid tetramers cat（r=0.999 6） and 0.205～5.74 μg for salnianolic acid B （r=0.999 7）.The average rec-overies of caffeic acid tetramer and salnianolic acid B were 98.72%（RSD=3.27%,n=9）and 101.39%（RSD=1.81%,n=9）,resp-ectively. CONCLUSION：The method is simple,reliable,stable and reproducible for quality control of Compound zicao granule.