不同转移潜能肿瘤细胞胸腺素β10表达水平及微丝结构的差异性研究

目的 研究胸腺素β10(Tlaymosinβ10,Tβ10)表达水平与人类肿瘤转移潜能的相关性以及细胞内微丝骨架的变化差异。方法 利用Northern-blot和细胞爬片免疫组织化学的方法检测4组9种具有不同转移潜能的人类肿瘤细胞系中Tβ10 mRNA和蛋白的表达水平;通过组织化学的方法显示细胞内微丝骨架的变化。结果 Tβ10的mRNA和蛋白表达水平在高转移潜能的人类肺癌、黑色素瘤和乳腺癌细胞中高于相应的不／低转移肿瘤细胞;高转移性癌与不／低转移癌细胞比较,前者细胞内肌动蛋白多聚体减少,微丝骨架杂乱、模糊,而低转移癌系微丝骨架粗大、结构清晰、排列整齐。结论 在所研究的人类肿瘤中,Tβ10 mRNA和蛋白表达水平与肿瘤的转移能力呈正相关性;肿瘤转移潜能的增高伴有肌动蛋白多聚体的丢失和微丝结构的解聚,微丝骨架的这种变化与Tβ10的表达增高具有相关性。

Thymosin beta10 expression and actin filament organization in tumor cell lines with different metastatic potential

OBJECTIVE: To investigate the expression of thymosin beta10 (Tbeta10) and related changes of actin filament organization in human tumor cell lines with different metastatic potential. METHODS: Four groups of nine human tumor cell lines with different metastatic potential were analyzed for the expression of Tbeta10 mRNA detected by northern-blot and its peptide by immunohistochemical staining. The filamentous actin (F-actin) was stained with TRITC-phalloidin to detect changes in actin organization. RESULTS: In comparison with the non and/or weakly metastatic counterparts, Tbeta10 was upregulated in highly metastatic human lung cancer, malignant melanoma and breast cancer cell lines. TRITC-phalloidin staining revealed less actin bundles and a fuzzy network of shorter filaments in the highly metastatic tumor cells, while in the non and/or weakly metastatic cancer cell lines, there were thick and orderly arranged actin filaments. CONCLUSIONS: Tbeta10 levels correlate positively with the metastatic phenotype in human tumors currently examined. The increased metastatic potential of tumor cells is accompanied by the loss of F-actin and poorly organized actin skeleton. There is a consistent correlation between the elevated Tbeta10 expression and the disrupted actin skeleton.