生物素标记组合探针杂交检测患儿尿液以诊断巨细胞病毒感染

利用 HCMV AD 169株的克隆 DNA 片段制成生物素标记的组合探针进行临床标本的 HCMV DNA 的杂交检测,此探针特异性强,不与其它类 DNA 发生非特异性杂交,可检出25pg 同源 DNA,100～500个 HCMV 感染细胞或2.57LgTCID50 HCMV 病毒悬液.杂交法结果与病毒分离结果符合率极高.初步用于临床尿标本检测得出,先天畸型,乳儿肝炎及激素治疗三组患儿中,HCMV 的排毒率分别为100%(3/3),61%(11/18)和75%(3/4).提示 HCMV 可能是临床部分患儿的重要致病源.同时表明杂交法对 HCMV 感染的检测是快速、特异及敏感的.

Detection of Clinical Human Cytomegalovirus Infection from Children by DNA Hybridisation with the Biotinylated Combined Probes

A dignostic assay has been developed to detect HCMV DNA in clinical specimens with Biotin-labelled combined probes which were prepared from some cloned fragments of HCMV strain AD169.The labelled probe can detect 25pg of HCMV homologous DNA and fail to hybridize with DNA from other sources.With excellent specificity,the combined DNA probes can detect 100～500 infected cells or 2.57lg TCID₅₀ of HCMV.The assay have high correlation when compared to virus isolation.In the detection of clinical speciments,it correctly identified 3(100%) of 3 urine specimens from congenital deformith positive children.11(61%) of 18 urine specimens from hepatitis positive baby,and 3(75%) of 4 urine specimens from Hormon treatment positive children.There is the evidence that the hybridization assay are specific,sensitive and rapid method for HCMV infection detection.