荧光定量PCR检测HBV DNA与HBV血清标志物的对比监测和分析

目的：探讨荧光定量PCR法检测乙型肝炎病毒(HBV)DNA与HBV血清免疫学标志物(HBVM)的相互关系。方法：801例血清标本采用ELISA方法对其免疫学标志物进行检测，并用实时荧光定量-聚合酶链反应(FQ-PCR)技术检测血清HBV DNA。结果：HBV DNA的总检出率为67.0％，在模式HBsAg( )，HBeAg( )，HBcAb( )中，HBV DNA的含量明显高于HBsAg( )，HBeAb( )，HBcAb( )及HBsAg( )，HBcAb( )模式。在HBsAg(-)模式中HBV DNA亦有检出。结论：HBVM能提供乙肝感染的间接证据，而荧光定量PCR法检测HBV DNA准确灵敏，能真实反映HBV感染、复制及病程变化，在乙型肝炎的诊断治疗中具有重要的临床指导价值。

Comparative Detection and Analysis of HBV DNA Tested by Quantitative Fluorescence PCR and HBV Serum Immune Markers

Objectve:To observe the relationship between HBV DNA tested by quantitative fluorescence PCR technique and HBV serum immune markers (HBV M). Methods: HBV M and HBV DNA was tested in 801 serum samples by ELISA and real time fluorescence quantitative PCR (FQ-PCR) respectively.Results: The total positive rate of serum HBV DNA was 67.0%. In the group with the positive HBsAg,HBeAg,and HBcAb, the contents of HBV DNA were significant greater than that of groups with positive HBsAg, HBeAb, HBcAb or HbsAg and HBcAb. HBV DNA was also tested to be positive in some of the serum with negative HBsAg.Conclusion:HBV M can give a proof of the infection of HBV indirectly. The quantitative fluorescence PCR determination of serum HBV DNA was correct and sensitive and could reflect the course of HBV infection. So it has an important value in the clinical diagnosis and cure of HBV.