Wnt信号通路成员APC抑癌基因在肾上腺皮质腺瘤中杂合性缺失的研究

目的：探讨APC基因杂合性缺失在。肾上腺皮质腺瘤中的意义。方法：采用聚合酶链反应-限制性酶切片段长度多态性（PCR—RFLP）方法研究Wnt信号通路成员APC基因11外显子RsaI酶切位点和15外显子MspI酶切位点多态性，分析42例肾上腺皮质腺瘤中APC基因杂合性缺失。结果：在42例肾上腺皮质腺瘤组中杂合子有30例（71．4％），未发现杂合性缺失。结论 肾上腺皮质腺瘤未发现APC基因杂合性缺失现象，据此认为APC基因杂合性缺失在肾上腺皮质腺瘤发生发展过程中可能无明显关联。

Loss of Heterozygosity of the Member of Wnt Signaling Pathways:APC Tumor Suppressor Gene in Adrenocortical Adenoma

Objectlve：To investigate the significance of the loss of heterozygosity （LOH） of adenomatous polyposis coli （APC） gene in adrenocortical adenoma. Methods. The polymorphism of Rsa I sites in the exonll and Msp I sites in the exonl5 of APC gene, the member of Wnt signaling pathways were studied by by using Polymer- ase Chain Reaction/ Restriction Fragment Length Polymorphism（PCR-RLFP）, LOH of adenomatous polyposis coil （APC） were analyzed in 42 adrenocortical adenomas. Results： In 42 adrenocortical adenoma samples, 30 were informative（30/42, 71.4%）, but none of them show LOH. Conclusions： None of the APC gene adrenocortical adenomas show LOH, it is supposed that the LOH of APC gene might not correlated with the development of adrenocortical adenomas.