Fabrication of a novel hybrid scaffold for tissue engineered heart valve |
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Authors: | Hao Hong Nianguo Dong Jiawei Shi Si Chen Chao Guo Ping Hu Hongxu Qi |
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Institution: | 1. Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 2. Department of Chemical Engineering, Institute of Polymer Science and Technology, Tsinghua University, Beijing 100084, China |
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Abstract: | The aim of this study was to fabricate biomatrix/polymer hybrid scaffolds using an electrospinning technique. Then tissue
engineered heart valves were engineered by seeding mesenchymal stromal cells (MSCs) onto the scaffolds. The effects of the
hybrid scaffolds on the proliferation of seed cells, formation of extracellular matrix and mechanical properties of tissue
engineered heart valves were investigated. MSCs were obtained from rats. Porcine aortic heart valves were decellularized,
coated with poly(3-hydroxybutyrate-co-4-hydroxybutyrate) using an electrospinning technique, and reseeded and cultured over
a time period of 14 days. In control group, the decellularized valve scaffolds were reseeded and cultured over an equivalent
time period. Specimens of each group were examined histologically (hematoxylin-eosin HE] staining, immunohistostaining, and
scanning electron microscopy), biochemically (DNA and 4-hydroxyproline) and mechanically. The results showed that recellularization
was comparable to the specimens of hybrid scaffolds and controls. The specimens of hybrid scaffolds and controls revealed
comparable amounts of cell mass and 4-hydroxyproline (P>0.05). However, the specimens of hybrid scaffolds showed a significant increase in mechanical strength, compared to the controls
(P<0.05). This study demonstrated the superiority of the hybrid scaffolds to increase the mechanical strength of tissue engineered
heart valves. And compared to the decellularized valve scaffolds, the hybrid scaffolds showed similar effects on the proliferation
of MSCs and formation of extracellular matrix. It was believed that the hybrid scaffolds could be used for the construction
of tissue engineered heart valves. |
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Keywords: | tissue engineered heart valve hybrid scaffold electrospinning mesenchymal stem cells |
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