| RNAi抑制皮肤鳞癌A431细胞EGFR表达的实验研究 |
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| 引用本文： | 吴波,郑锦芬,余振东,江智茂,马刚.RNAi抑制皮肤鳞癌A431细胞EGFR表达的实验研究[J].第四军医大学学报,2009,30(12). |
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| 作者姓名： | 吴波 郑锦芬 余振东 江智茂 马刚 |
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| 作者单位： | 吴波,郑锦芬,马刚,WU Bo,ZHENG Jin-Fen,MA Gang(北京大学深圳医院皮肤科,广东,深圳,518036);余振东,YU Zhen-Dong(北京大学深圳医院检验科,广东,深圳,518036);江智茂,JIANG Zhi-Mao(北京大学深圳医院中心实验室,广东,深圳,518036)
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| 摘 要： | 目的:研究RNA干扰(RNA interference,RNAi)对皮肤鳞癌A431细胞表皮生长因子受体(EGFR)基因表达的抑制作用及其对A431细胞增殖、凋亡的影响.方法:设计制备两对针对EGFR基因的小干扰RNA(small interfering RNA,siRNA),转染A431人皮肤鳞癌细胞株,采用RT-PCR法检测EGFR mRNA的表达;Western Blot测定EGFR蛋白的表达;MTT法检测细胞生长的影响;流式细胞仪检测细胞凋亡率.结果:siRNA2干扰后EGFR mRNA与蛋白的表达强度分别为(16.5±1.6)%和(11.1±1.6)%,与对照组比较有统计学差异(P<0.05);在EGFR表达受到抑制的同时,转染后的A431胞生长速度明显变慢;A431细胞的凋亡率为(12.6±1.4)%,与对照组比较有统计学差异(P<0.05).结论:siRNA2能有效抑制皮肤鳞癌细胞EGFR基因的表达,抑制细胞的增殖,并促进细胞凋亡.
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| 关 键 词： | RNA干扰 表皮生长因子受体 鳞状细胞癌 细胞凋亡 |
Inhibitory effects of siRNA on expression of epidermal growth factor receptor in squamous cell carcinoma cell line A431 |
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| WU Bo,ZHENG Jin-Fen,YU Zhen-Dong,JIANG Zhi-Mao,MA Gang.Inhibitory effects of siRNA on expression of epidermal growth factor receptor in squamous cell carcinoma cell line A431[J].Journal of the Fourth Military Medical University,2009,30(12). |
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| Authors: | WU Bo ZHENG Jin-Fen YU Zhen-Dong JIANG Zhi-Mao MA Gang |
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| Institution: | WU Bo1,ZHENG Jin-Fen1,YU Zhen-Dong2,JIANG Zhi-Mao3,MA Gang11Department of Dermatology,2Department of Clinical Laboratory,3Department of Central Laboratory,Shenzhen Hospital Peking University,Shenzhen 518036,China |
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| Abstract: | AIM: To investigate the inhibitory effect of RNA interference(RNAi) on the epidermal growth factor receptor(EGFR) gene expression in A431 cell line.METHODS: Two pairs of small interfering RNA(siRNA) for EGFR gene were designed and transfected into A431 cells.EGFR gene expression was assessed by RT-PCR and EGFR protein expression was tested by Western blot.The proliferation of the A431 cells was detected by MTT method and flow cytometry was used to measure the cell apoptosis.RESULTS: The expression rates of ... |
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| Keywords: | RNA interference epidermal growth factor receptor squamous cell carcinoma apoptosis |
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