慢性间断缺氧对老年期大鼠脑白质的影响

目的研究慢性间断缺氧对老年期大鼠脑白质的影响。方法建立老年期SD大鼠慢性间断缺氧模型，免疫组化检测大鼠脑室旁白质髓鞘碱性蛋白（myelin basic protein，MBP）、神经微丝H＋L（neutofilament—H＋L，NF-H＋L）、胶质纤维酸性蛋白（glial fibrillary acidic protein，GFAP）表达并行图像分析；电镜下观察大鼠脑内髓鞘、轴突的超微结构。结果慢性间断缺氧可使老年期大鼠脑白质MBP、NF-H＋L表达减少及GFAP表达增加（P均〈0．05），MBP与NF-H＋L呈高度正相关（R^2=0．908，P〈0．01）。电镜观察与空白对照组比较，缺氧组大鼠脑内可见较多的髓鞘脱失、轴突受损。结论慢性间断缺氧可对老年期大鼠脑白质产生不良影响，表现为髓鞘脱失、轴突变性以及神经胶质增生。

The influence of chronic intermittent hypoxia in the senile period on the cerebral white matter of the rats

Objective To study the influence of chronic intermittent hypoxia in the senfle period on the cerebral white matter of the rats. Methods The model of chronic intermittent hypoxia of the elderly S-D rats was established. Immunohistoehemistry was used to detect the expression of myelin basic protein（MBP）, neurofilament-H ＋ L（NF-H ＋ L）, and glial fibrillary acidic protein（GFAP） in the white matter near the lateral ventricle of the rats. The pathological image analysis system was used to determine the quantity. Mierostructures of the myelin and axon in the rat brain were observed by electron microscope. Results Chronic intermittent hypoxia in the senile period reduced the expression of MBP and NF-H ＋ L, and increased the expression of GFAP in the white matter of the rats（P〈0. 05）. Moreover, there was a high positive correlation between the expression of MBP and NF-H ＋ L in the white matter near the lateral ventricle（R^2 = 0. 908, P〈0. 01 ）. More demyelination and injured axon were observed by the electron microscope in the hypoxia group than that in the control group. Conclusions Chronic intermittent hypoxia in the senile period brings harmful effects to cerebral white matter of the rats, which may increase the risk of demyelination, injured axon and astroglia proliferation.