口服丙烯酰胺对雄性大鼠生长发育及生殖机能的影响

目的:研究丙烯酰胺对雄性大鼠的生殖毒性作用。方法:30只21日龄断奶未成熟雄性大鼠随机分为3组,实验组Ⅰ和实验组Ⅱ分别通过自由饮水方式口服5 mg/kg.d和10 mg/kg.d的丙烯酰胺溶液8周,对照组饮用自来水。分两批(第4周和第8周时)对体重、脏器重等指标进行检测,并做睾丸和附睾的组织形态学观察;第8周时,同时检查附睾尾精子密度和精子形态。结果:两实验组大鼠体重增加显著低于对照组(P<0.05),至实验8周时,睾丸、附睾性器官发育已受到影响,实验组Ⅱ大鼠附睾尾部精子密度明显低于对照组(P<0.05),实验组Ⅰ与对照组差异不显著(P>0.05)。睾丸出现不同程度的病理变化,发生调亡的生精小管周围间质细胞显著增多(P<0.05)。结论:丙烯酰胺会对生精小管产生毒性作用而导致雄性大鼠精子生成减少。

Oral Acrylamide Affects the Development and Reproductive Performance of Male Rats

OBJECTIVE: The content of acrylamide increases remarkably in fried, baked and heat-processed starchy foods. The present experiment was aimed at investigating the toxicity of acrylamide on the reproductive system in male rats. METHODS: Thirty weaned 21-day-old SD male rats were randomly allotted into three groups of 10 each. Group I and Group II were fed on water solutions containing acrylamide 5 mg/kg/d and 10 mg/kg/d for eight consecutive weeks, while the third group on fresh water only as the control. The body weight, viscera weights and testicle and epididymis tissues were detected at the fourth and eighth week respectively. In the end of the experiment, the sperm reserve and morphology in the cauda of the epididymis were examined. RESULTS: The growth of the rats treated with acrylamide was retarded (P < 0.05). The weights of the testis and epididymis and the sperm concentration in the cauda of the epididymis of Group II were decreased significantly (P < 0.05) after acrylamide administration, while no significant change was observed in the sperm concentration of Group I (P > 0.05). Furthermore, histopathological lesions were presented in the testes of the treated rats, and the number of Leydig cells around the apoptosis seminiferous tubules increased significantly (P < 0.05). CONCLUSION: Acrylamide has toxic effects on seminiferous tubules and decreases the production of sperm in male rats.