Small‐animal PET imaging analysis with [18F]FHBG in a mouse model of HSV1‐tk gene expression in melanoma |
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| Authors: | Onseok Lee Jaeyoung Kim Chilhwan Oh |
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| Institution: | 1. Department of Medical IT Engineering, College of Medical Sciences, Soonchunhyang University, Asan City, Chungnam, Korea;2. Research Institute for Skin Image, Korea University College of Medicine, Guro‐gu, Seoul, Korea;3. Department of Dermatology, Korea University Guro Hospital, Korea University College of Medicine, Guro‐gu, Seoul, Korea |
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| Abstract: | The purpose of this study was to establish a small‐animal model for molecular imaging and to acquire basic data on assessing the efficacy of candidate melanoma drugs using small‐animal PET imaging analysis with 18F]FHBG for herpes simplex virus 1‐thymidine kinase (HSV1‐tk) gene expression in a melanoma mouse model. The B16 melanoma cell line was transduced with a recombinant lentiviral vector containing the HSV1‐tk gene and inoculated into the back skin of C57BL/6J mice. 18F]FHBG PET imaging showed better contrast for HSV1‐tk(+) melanomas compared to brain, heart, gall bladder, intestine and kidney than did 18F]FDG PET imaging. |
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| Keywords: | [18F]FDG [18F]FHBG HSV1‐tk lentiviral vector melanoma small‐animal PET/CT |
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