中药小蓟不同药用部位体外抗炎、促凝血的作用研究

目的：研究小蓟不同部位化学成分的差异与抗炎、促凝血病症疗效关系，研究生药不同部位的精准应用。方法：使用HPLC色谱法建立不同部位的指纹图谱，MTT法测定不同部位提取物对炎症细胞RAW264.7增值的抑制作用，ELISA法测定不同部位提取物对小鼠凝血酶原（PT）指标的影响，并使用灰色关联软件，将色谱峰的峰面积与抗炎、凝血酶原指标相关联，得到各个成分的贡献率。结果：小蓟根、茎、叶、花中的成分种类与含量均有不同。在体外抗炎药效研究中，小蓟花的效果最好，对炎症细胞RAW264.7 的抑制率达到63.25%。小蓟根次之，小蓟叶与茎对炎症细胞的抑制作用不大。用ELISA法测定小鼠血浆中凝血酶原指标，小蓟花对指标影响最大，叶次之，茎与根对指标变化影响不大。灰色关联度计算软件结果显示，绿原酸、橙皮苷、咖啡酸、蒙花苷对抑制炎症细胞增值（抗炎）的相关程度分别为0.7250，0.5249，0.5105，0.5096。与升高凝血酶原指标（止血）的相关程度分别为0.5982，0.5088，0.5037，0.5034。其关联程度与其他成分相比，差距较大。结论：小蓟不同部位和抗炎、促凝血病症的药效存在差异，明确了生药小蓟不同部位的精准应用。

Anti-inflammatory and Coagulant Effects Study of Different Medicinal Parts of Traditional Chinese Medicine Cirsium setosum

Objective: To study the difference of chemical constituents and pesticide effects of anti-inflammation and promote coagulation in different parts of Cirsium setosum, and set up the HPLC fingerprint of different parts of Cirsiumsetosum. Methods: The MTT assay was used to test the inhibitory action to the RAW264.7 cells of different parts ofCirsium setosum and use the ELISA assay to measure the effects of extracts from different parts on prothrombin (PT) inmice. Then use the GreyModeling V3.0 software to calculate the correlation coefficients of peak area of chromatographicpeaks and the pesticide effects. Results: Different species and contents of components in the roots, stems, leaves, andflowers are different in Cirsium setosum. The flowers of Cirsium setosum had the best effects on inhibition of RAW 264.7cells which the inhibition rate was 63.25%. The leaves of Cirsium setosum ranked second. The prothrombin in mice wasdetermined by ELASA assay. The flowers of Cirsium setosum had the greatest impact on the indicator. The roots ofCirsium setosum ranked second. The results of GreyModeling software shows that the chlorogenic acid, hesperidin, caffeicacid, linarin have greater degrees of contribution on anti-inflammation and promote coagulation which compared withothers. The correlation coefficients of four components on anti-inflammatory were 0.7250, 0.5249, 0.5105 and 0.5096, on coagulant effects were 0.5982, 0.5088, 0.5037, and 0.5034. Conclusion: Different groups of components in different partsof Cirsium setosum may prove to be the major factor to the different pesticide effects.