苎麻根石油醚部位体外抗肿瘤生物活性及其GC-MS分析

目的:明确苎麻根石油醚部位体外抗胃癌活性并揭示其发挥药效的物质基础,为苎麻根的开发利用奠定基础。方法:采用噻唑蓝(MTT)法考察不同给药剂量,不同给药时间下苎麻根石油醚部位对人胃癌HGC-27细胞的增殖抑制作用及其与时间、剂量的关系;采用流式细胞仪检测苎麻根石油醚部位作用于人胃癌HGC-27细胞后细胞凋亡率和细胞周期的变化;通过GC-MS对发挥药效的苎麻根石油醚部位进行成分解析。结果:不同质量浓度的苎麻根石油醚部位作用于人胃癌HGC-27细胞24,48,72 h后,均呈现出较好的细胞增殖抑制作用,并呈现一定的时间-剂量-效应关系,差异有统计学意义(P 0. 05);苎麻根石油醚部位作用人胃癌HGC-27细胞后可诱导细胞凋亡,影响细胞周期的正常变化,G0/G1期细胞比例明显减少,S期细胞比例明显增多;通过GC-MS鉴定出苎麻根石油醚部位中26种化学成分,主要包括谷甾醇、豆甾醇等。结论:苎麻根石油醚部位为其抗胃癌活性部位,其发挥药效的主要成分为甾醇类化合物,为苎麻根在临床上的合理应用及其在抗肿瘤方面的进一步研究奠定基础。

In Vitro Antitumor Bioactivity and GC-MS Analysis of Petroleum Ether Fraction of Boehmeria nivea Root

Objective:To define the anti-gastric cancer activity in vitro of petroleum ether fraction of Boehmeria nivea root and reveal the material basis of its efficacy, so as to lay the foundation for the development and utilization of B. nivea root. Method:Methyl thiazolyl tetraolium(MTT) method was used to evaluate the inhibitory rate and time-dose relationship of petroleum ether fraction of B. nivea root with different doses and delivery times on human gastric cancer HGC-27 cells. Flow cytometry was used to detect the change of cell apoptosis and cell cycle after petroleum ether fraction of B. nivea root acted on human gastric cancer HGC-27 cells. GC-MS was used to detect the components of petroleum ether fraction of B. nivea root. Result:Experiment data showed significant cell proliferation inhibition in an obvious time-dose-effect manner, with statistically significant differences (P<0.05),after 24,48,72 h of incubation of human gastric cancer HGC-27 cells with different concentrations of petroleum ether fraction of B. nivea root. The effect of petroleum ether fraction of B. nivea root on human gastric cancer HGC-27 cells could induce apoptosis,which affects the normal changes of cell cycle. The percentage of cells was decreased significantly in G₀/G₁ phase,and that in S phase was significantly increased. GC-MS was used to identify 26 chemical constituents in petroleum ether of B. nivea root,including sitosterol and stigmasterol. Conclusion:Petroleum ether fraction of B. nivea root is the active anti-gastric cancer part,and its main effective component is sterol compounds. This lays the foundation for the rational application of B. nivea root in clinic and the further research in tis anti-tumor effect.