基于正交投影偏最小二乘法对复方首乌藤合剂抗失眠作用的谱效关系研究

目的 探讨复方首乌藤合剂抗失眠作用与HPLC指纹图谱间的相关性。方法 采用HPLC分析得到不同提取工艺条件下的复方首乌藤合剂指纹图谱；腹腔注射DL-4-氯苯丙氨酸建立大鼠失眠模型，用ELISA法测定大鼠海马体5-HT的含量，采用正交投影偏最小二乘法分析谱效关系。结果 从指纹图谱中共确定21个共有峰，12个峰的变量投影重要性（VIP）均>1.0，复方首乌藤合剂指纹图谱中3个色谱峰对抗失眠发挥重要作用。结论 复方首乌藤合剂抗失眠作用的物质基础与其所含多种成分共同作用的结果有关，通过谱效关系分析得到复方首乌藤合剂的抗失眠物质基础，可为制剂的质量控制提供实验依据。

Study on spectrum-effect relationship of sedative and hypnotic effect of compound caulis polygoni multiflori mixture based on orthogonal partial least squares regression analysis

Objective To investigate the correlation between anti-insomnia effects and HPLC fingerprint of compound caulis polygoni multiflori mixture.Methods A fingerprinting method was established by high performance liquid chromatography and then used to analyze different batches of compound caulis polygoni multiflori mixture.Rat insomnia model was established by intraperitoneal injection of p-chlorophenylalanine,5-HT in rat hippocampus was determined by ELISA.Then the orthogonal partial least squares (OPLS)analysis was used to investigate the spectrum-effect relationship.Results 21 common peaks were identified from the fingerprints,the variable projection importance (VIP)values of these 12 peaks were more than 1.0,indicated that the 3 peaks had played an important role in the anti-insomnia activity of compound caulis polygoni multiflori mixture.Conclusion The anti-insomnia activity of compound caulis polygoni multiflori mixture was the result of synergistic effect of multiple components,its material basis could be analyzed by spectrum-effect relationship,which could provide basis for the quality control of preparation.