灰色关联度法研究红蓼抗肿瘤的药用部位研究

目的：考察不同药用部位红蓼对肝癌细胞的增殖抑制作用；确定红蓼抗肝癌的最优药用部位。方法：采用高效液相色谱法建立多批次红蓼根、茎、叶、花的指纹图谱；采用MTT法分别对其抑制癌症细胞增值药效进行研究；并使用灰色关联软件计算贡献程度；计算多批次红蓼不同药用部位抗肝癌的平均值。结果：不同药用部位红蓼均能够抑制肝癌细胞生长；灰色关联度分析结果显示红蓼抑制肝细胞关联系数≥ 0.7的高效液相指纹峰为10（槲皮素）、13、14（山萘酚）、17号；6批红蓼根、茎、叶、花、果抗肝癌抑制率为32.23%、34.55%、38.48%、44.39%和42.27%。结论：红蓼各药用部位均有抗肝癌的作用，其中槲皮素和山萘酚为红蓼各药用部位发挥抗肝癌的物质基础。

Study on the Antitumor Precise Drug Sites of Polygonum Orientale Based on Grey Relational Degree Method

Objective: To investigate the inhibitory effect of Polygonum orientale on the proliferation of hepatocellular carcinoma cells and determine the optimal medicinal site of Polygonum orientale against hepatocellular carcinoma.Methods: HPLC method was used to establish the fingerprint of Polygonum orientale??s root, stem, leaf and flower. MTTassay was used to study its inhibitory effect on cancer cell proliferation. Gray correlation software was used to calculatethe contribution of fingerprint peak. The mean values of Polygonum orientale against hepatocellular carcinoma werecalculated. Results: Polygonum orientale could inhibit the growth of hepatocellular carcinoma cells. The results of greycorrelation analysis showed that the fingerprint peaks of Polygonum orientale inhibition hepatocyte correlation coefficientgreater than or equal to 0.7 were No.10, 13, 14 and 17. The mean values of anti-hepatocellular carcinoma were 32.23%,34.55% , 38.48% , 44.39% and 42.27% . Conclusion: All the medicinal parts of Polygonum orientale had antihepatocellularcarcinoma, among which quercetin and sanaphthol were the basis of anti-hepatocellular carcinoma.