内皮抑素对人卵巢癌细胞系生长的抑制作用

目的探讨人内皮抑素对人卵巢癌SKOV3细胞生长的抑制作用及其作用机制。方法MTT法检测细胞生长;透射电镜观察细胞凋亡;免疫细胞化学、RT-PCR及Western blot法检测细胞中BCL-2和BAX蛋白及mRNA的表达。结果内皮抑素抑制SKOV3细胞增殖(P<0.01);能诱导SKOV3细胞凋亡;而对细胞中BCL-2和BAX蛋白及mRNA的表达无明显影响。结论人内皮抑素具有抑制卵巢癌细胞SKOV3生长的作用,其作用机制可能与诱发细胞凋亡相关。

Inhibition of endostatin on growth of ovarian cancer cell line SKOV3

Objective To evaluate the inhibitory effect of endostatin on ovarian cancer cell line SKOV3 and to investigate the possible mechanism of the inhibition.Methods The effect of endostatin on SKOV3 cell proliferation was studied by means of MTT.The cell apoptosis was detected by transmission electronic microscopy.BCL-2 and BAX expressions were measured in SKOV3 cells treated by endostatin by immunocytochemistry,RT-PCR and Western blot.Results Endostatin inhibited SKOV3 cell proliferation(P<0.01) and induced cell apoptosis,whereas BCL-2 and BAX expression were not changed obviously in SKOV3 cell treated by endostatin.Conclusion Endostatin may have antitumor effects on ovarian cancer cell SKOV3 and that at least in part,one of the important mechanisms of endostatin action on the potent antiangiogenic and antitumor activities may induce apoptosis.