健脾补肾方对615小鼠前胃癌术后复发瘤细胞凋亡和线粒体膜电位的影响

目的：研究健脾补肾方对小鼠前胃癌术后复发瘤细胞凋亡和线粒体膜电位的影响。方法：以615小鼠前胃癌术后模型为对象,应用健脾补肾方,观察抑制术后复发的作用,应用流式细胞仪检测复发瘤的细胞凋亡的水平和线粒体膜电位。结果：与模型组比较,健脾补肾组复发瘤瘤重显著降低,复发瘤抑制率为71.07%;复发瘤凋亡指数显著增高：复发瘤细胞线粒体膜电位（MMP）显著降低。以上各项指标均P〈0.01。结论：健脾补肾方对术后局部肿瘤复发有明显的抑制作用,导致复发瘤细胞线粒体膜电位降低,从而诱导肿瘤细胞凋亡。

Effect of Jianpi Bushen Formula on the Apoptosis and Level of Mitochondrial Membrane Potential in 615 Murine with Cancer Cell Line MFC after Surgical Operation

Objective:To study the inducing effect of Jianpi Bushen Formulation(JPBS) on apoptosis and the level of mitochondrial membrane potential(MMP) in 615 murine with MFC after surgical operation.Methods:615 murine with MFC after surgical operation was adopted as animal model.JPBS were orally delivered to the animals for therapy.The effects were evaluated by inhibition of local recurrence tumor.Apoptosis and the level of MMP of those was detected respectively using flow cytometry(FCM).Results:After treatment,the weight of recurrence tumor of JPBS group was obviously lower than that of model group.The inhibition rate of recurrence tumor of JPBS group was 71.07%.Apoptotic index of JPBS group was obviously higher than that of model group.The MMP level of JPBS group was obviously lower than that of model group.(all P<0.01) Conclusions:JPBS were effective for local recurrence tumor after resection of primary tumor.The MMP level of local recurrence tumor may be degraded,and apoptosis of that my be induced by JPBS.