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| 大黄素对人胆管癌多药耐药细胞QBC_（939）/ADM的耐药逆转作用 | |
| 引用本文： | 方志强,张萍,米志坚,王世明. 大黄素对人胆管癌多药耐药细胞QBC_（939）/ADM的耐药逆转作用[J]. 中国现代药物应用, 2009, 3(8): 31-33 |
| 作者姓名： | 方志强 张萍 米志坚 王世明 |
| 作者单位： | 1. 山西医科大学第一医院普外科,030001 2. 太原市疾病预防控制中心免疫规划科 3. 山西职工医学院解剖教研室 |
| 摘 要： | 目的探讨大黄素（Emodin,EM）对人胆管癌多药耐药细胞QBC939/ADM的耐药逆转作用。方法细胞生长周期、凋亡率采用流式细胞术（FCM）,多药耐药P糖蛋白（P-gP）以免疫组织化学（SP）法检测。结果阿霉素（ADM）、紫杉醇分别与EM联合作用于人胆管癌多药耐药细胞QBC939/ADM的凋亡率比单独作用有显著提高。细胞周期分布显示：ADM、紫杉醇分别与EM联合作用于人胆管癌多药耐药细胞QBC939/ADM,使QBC939/ADM阻滞于G2/M期的细胞数明显增加。EM和紫杉醇联合作用后,人胆管癌多药耐药细胞QBC939/ADM中多药耐药P糖蛋白（P-gP）的表达由72.00%降低到46.00%（P〈0.01）。结论EM对QBC939/ADM细胞的多药耐药性有逆转作用。其作用机理可能与人胆管癌细胞P-gP的表达有关,通过与化疗药物竞争性结合P-gP的结合位点发挥疗效。 |
| 关 键 词： | 多药耐药 大黄素 P糖蛋白 |
Reversal effect of Emodin on the human cholangiocarocinoma multidrug resistance cell-QBC939/ADM |
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| Affiliation: | FANG Zhi-qiang, ZhANG Ping, MI Zhi-jian, et al. (Dep of general surgery, First Clinical Medical College, Shanxi Medical University, Taiyuan, Shanxi 030001, China) |
| Abstract: | Objective To explore the reversal effect of emodin （EM） on the drug resistance of multi- drug resistance cell line QBC939/ADM. Methods Cell cycle and apoptosis of above-mentioned each group ceils were performed by flow cytometry （FCM）. And P-glycoprotein was measured by immunohistochemistry.Results The apoptosis rate of the cells was significance different that EM, ADM and Paclitaxel were combined used to QBC939/ADM than single used. The distribution in cycle of cells show Cells of G2/M phase increased. The influence of growth cycle was obvious in two groups. The levels of P-glycoprotein （P-gp） on the cells of QBC939/ ADM were from 72.00% to 46. 00% （P 〈 0. 01 ） . Conclusion EM can reverse the multi-drug-resistant of human bile duct carcinoma cell line （QBC939/ADM）. That may have something to do with the over-expression of the P-gp. |
| Keywords: | Multidrug resistance Emodin P-glycoprotein |
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