四君子汤对两种脾虚模型大鼠肠道菌群紊乱的影响

目的 观察两种脾虚模型大鼠肠道菌群的变化，研究四君子汤对肠道菌群紊乱的影响。

Effect of Sijunzi Decoction on the Intestinal Flora Disturbance in Two Rat Models of Pi-deficiency Syndrome

Objective To investigate the changes of intestinal microflora in two rat models of Pi-deficiency syndrome（PDS） to study the effect of Sijunzi Decoction（SJZD） on intestinal microflora alteration（IMA）.Methods Wistar rats allocated in Group A and B were established into two kinds of PDS models by Folium Sennae and Radix et Rhizoma Rhei respectively.Fecal samples were collected from the model rats at three phases,i.e.before modeling,after modeling and after treated with SJZD.Total DNA of microbial communities in them was extracted and amplified with ERIC-PCR to generate fingerprints for reflect the intestinal microflora configuration.Shannon-Weaver index（H′） was used to analyze the diversity of rats′ intestinal microflora,Soreason pairwise similarity coefficient（Cs） was used to compare the similarity of different ERIC-PCR fingerprints,and Student′s t test was used for analyzing the statistical significance of their differences.Results The intestinal microflora in both groups obviously changed after modeling and recovered to the primary level after treated with SJZD.The H′ of the ERIC-PCR fingerprints at the three phases was 2.20±0.12,2.00±0.10 and 2.27±0.10 in Group A,and 2.09±0.02,1.88±0.10 and 1.94±0.10 in Group B respectively,both showing significant differences among different phases（P〈0.05）.Accumulative curves of Cs showed that the proportion of Cs accounting for less than 60% was 0 in Group A and 19% in Group B before modeling,75% and 52% for them respectively after modeling,and 4% and 24% after rehabilitation with SJZD.Conclusion The H′ of intestinal microflora in the two kinds of PDS models were significantly lowered,and could be recovered to the normal level by SJZD treatment（P〈0.05）.ERIC-PCR fingerprints can be used as a bio-criterion for evaluating intestinal microflora in patients with PDS.