高效液相色谱荧光法测定Beagle犬血浆中酒石酸美托洛尔对映体浓度

目的:建立Beagle犬血浆中酒石酸美托洛尔对映体浓度的高效液相色谱荧光测定方法。方法:Beagle犬口服消旋体酒石酸美托洛尔100mg后取1·5h血样进样测定,手性色谱柱为ChiralcelOD-H,流动相为正己烷-异丙醇-二乙胺(65∶35∶0·1),荧光检测波长Ex和Em分别为267nm、290nm,柱温为25℃,流速为0·6ml/min,内标物为(S)-阿替洛尔。结果:酒石酸美托洛尔2种对映体检测浓度在10~2000ng/ml范围内线性关系良好,平均回收率为96·12%~116·9%(RSD<7·6%)。结论:该分析方法选择性好、准确性高、重现性好,适于酒石酸美托洛尔对映体的药动学研究。

Determination of Metoprolol Tartrate Enantiomers in Beagle Dogs by HPLC Fluorescence Method

OBJECTIVE:To establish HPLC method for the determination of plasma metoprolol tartrate enantiomers in Beagle dogs.METHODS:Plasma samples were taken from Beagle dogs at1.5h after oral administration of metoprolol tartrate100mg,Chiralcel OD-H was taken as the chiral chromatographic column,the mobile phase consisted of N-hexane-dimethyl carbinol-diethylamine(65∶35∶0.1)with a flow rate at0.6ml/min,the fluorescence detection wavelength Ex was267nm and Em was290nm,the column temperature was25℃and the internal standard was(S)-atenolol.RESULTS:Good linear re-lationship was achieved when the detection concentration of metoprolol enantiomers was within the range of10～2000ng/ml,the average recovery was96.12%～116.9%(RSD<7.6%).CONCLUSIONS:The method is selective,accurate and reproducible,and suitable for the pharmacokinetics study of metoprolol tartrate enantiomers.