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| 高效液相色谱法测定注射用卡络磺钠原料药的含量 | |
| 引用本文： | 陈莉,崔颖,段姚尧.高效液相色谱法测定注射用卡络磺钠原料药的含量[J].武警医学院学报,2008,17(2):90-92. |
| 作者姓名： | 陈莉 崔颖 段姚尧 |
| 作者单位： | 1. 武警医学院,药剂教研室,天津,300162 2. 武警医学院,药化教研室,天津,300162 3. 武警医学院,科研部,天津,300162 |
| 摘 要： | 【目的】建立高效液相色谱法测定注射用卡络磺钠原料药含量,对其质量进行控制。【方法】采用Hy-persilC18柱为分析柱,以0.02 M磷酸二氢铵缓冲液-乙醇（90∶10）为流动相,流速1 ml/min,紫外检测器检测,检测波长360 nm。【结果】在2.0~60μg/ml浓度范围内,卡络磺钠的峰面积与浓度呈线性关系,相关系数为0.9999;方法平均回收率为100.6%,RSD为0.85%。【结论】本法准确、方便,可用于测定注射用卡络磺钠原料药含量。 |
| 关 键 词： | 高效液相色谱法 卡络磺钠 原料药 |
| 文章编号： | 1008-5041(2008)02-0090-03 |
| 修稿时间： | 2007年7月14日 |
Determination on the content of injection carbazochrome sodium sulfonate by HPLC |
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| CHEN Li,CUI Ying,DUAN Yao-yao.Determination on the content of injection carbazochrome sodium sulfonate by HPLC[J].Acta Academiae Medicinae CPAPF,2008,17(2):90-92. | |
| Authors: | CHEN Li CUI Ying DUAN Yao-yao |
| Institution: | CHEN Li;CUI Ying;DUAN Yao-yao(Department of Pharmacy;Medical College of Chinese People′s Armed Police Force;Tianjin 300162;China) |
| Abstract: | 【Objective】To establish a method to determine the content of carbazochrome sodium sulfonate in injection with HPLC.【Methods】The assay was conducted on a Hypersil-C18 column with 0.12% NH4H2PO4 buffer-alcohol（90∶10）as mobile phase and the detection wave length was set at 360 nm.【Results】The linear concentration range of carbazochrome sodium sulfonate was 2.0～60 μg/ml with the correlation coefficient of 0.9999 and the average recovery was 100.6%（RSD=0.85%）.【Conclusions】The method is accurate and simple and can be used for the determination of the raw material of carbazochrome sodium sulfonate used in injection. |
| Keywords: | HPLC Carbazochrome sodium sulfonate Row material |
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