Ala-Gln和BCAA对创伤大鼠静脉营养支持作用的实验研究

目的观察富含丙氨酰谷氨酰胺(Ala—Gln)和支链氨基酸(BCAA)的氨基酸注射液对创伤大鼠的静脉营养支持效果。

Effect of Ala-Gln and BCAA on traumatized rat

Purpose To observe the effects of amino acids solution supplimenteel with alanylglutamine (Ala-Gln) and branched-chain amino acids (BCAA) on traumatized rats. Methods After the injuny, and centeral vena catheter placed, 40 male, Wistar rats were randomly divided into nitrogen-free, normal diet, control and experimental groups. Dietary intake was weighed everyday, and body weight was checked every other day. On days 4 and 8, nitrogen balance was measured. On days 14, serum proteins and amino acids concentration and peripheral lymphocyte blastogenesis were determined. At the end of the study, the animals were sacrificed, hydroxyproline and tensile strength of wound, and intestinal mucosal DNA, RNA and protein content were examined. Results Nitrogenfree group was worse in all variance than other groups. The nitrogen balance on days 8 was remarkably improved in experimental and normal diet groups compared with control group. There were highter serum glutamine (Gln) and BCAA concentration, hydroxyproline content and tensile strength of wound, and better lymphocyte blastogenesis in experimental group compared with control and normal diet groups. Experimental and normal diet groups had higher mucosal DNA, RNA and protein content than control group. Conclusions Gin and BCAA can optimize hypercatabolism, enhance immunity, and promote wound healing and the growth of intestinal mucosa.