山楂总黄酮对人血管内皮细胞的作用

目的 探讨山楂总黄酮对人血管内皮细胞的保护作用及其机理.方法 通过原代培养人脐静脉内皮细胞(EC),从细胞形态学、细胞生长状况、细胞内乳酸脱氢酶(LDH)释放、内皮细胞对单核细胞(MC-EC)的粘附作用,以及低密度脂蛋白脂质过氧化(TBARS值)测定等,观察不同剂量(25,50,100mg/L)山楂总黄酮对氧化型低密度脂蛋白(oxLDL)诱导的EC损伤的影响和对EC的直接作用.结果 oxLDL对EC具有明显的损伤作用,oxLDL组的细胞生存率低于正常对照组(P<0.05),LDH释放率和MC-EC粘附率均高于正常对照组(P<0.05);山楂黄酮可以有效地抑制oxLDL对EC的损伤,3个剂量的山楂黄酮+oxLDL组的细胞生存率均显著高于oxLDL组(P<0.05),LDH释放率、MC-EC粘附率和TBARS值均显著低于oxLDL组(P<0.05),呈剂量-效应关系.单纯山楂黄酮组的中、高剂量组MC-EC粘附率均显著低于正常对照组(P<0.05).结论 山楂黄酮可以有效地保护内皮细胞免受oxLDL的损伤,其机理与山楂黄酮的抗氧化作用和对EC的直接作用有关.

Effects of Haw Flavone on Human Vascular Endocelial Cells

ve To examine the protective effect and its mechanism of haw thorn flavone on human vascular endo-celial cells. Methods Original human umbilical vein endocelial cells(EC) were cultured, and the changes of cell morphology, cell grow condition, lactate dehydragenase (LDH) released from cells, monocyte(MC) adhesion to EC, and thiobarbituric acid-reaction substance (TBARS) were measured when EC was incubated with oxidzed low density lipoprotein (oxLDL, 100 mg/1) in the presence or not of haw thorn flavone, as well as with single haw flavone at three different doses(25,50, 100 mg/1). Results EC survive rate of oxLDL group was lower (P<0.01) and LDH release rate was higher(P < 0.05) than those of normal control group. Haw thorn flavone could protect EC from damage caused by oxLDL. The EC survive rates of haw thorn flavone + oxLDL groups in three doses were higher(P < 0.05), the LDH release rates, MC-EC sticking rates and TBARS were lower( P< 0.05) in a dose-effect manner compared with those of oxLDL group, respectively. The MC-EC sticking rates of single haw flavone groups in medium and high dose were lower than those of normal control group(P<0.05) .Conclusion Haw thorn flavone could effectively protect EC from damage induced by oxLDL, which was contributed to its antioxidation activity and direct work on EC.