复方丹参滴丸对视网膜缺血再灌注损伤模型大鼠的疗效观察

目的　探究复方丹参滴丸对大鼠视网膜缺血再灌注损伤（ｒｅｔｉｎａｌｉｓｃｈｅｍｉａｒｅｐｅｒｆｕｓｉｏｎｉｎｊｕｒｙ，ＲＩＲＩ）的影响。方法　将４２只健康成年ＳＤ大鼠随机分为正常对照组（６只）、模型组（１８只）和治疗组（１８只）。模型组和治疗组采用前房灌注加压建立大鼠ＲＩＲＩ模型，治疗组ＲＩＲＩ后立即给予复方丹参滴丸（４５０ｍｇ?ｋｇ－１?ｄ－１）灌胃，连续７ｄ，模型组和正常对照组灌胃等量生理盐水。观察ＲＩＲＩ后２４ｈ、７ｄ视网膜电流图的改变，并取视网膜行组织病理学观察。测量ＲＩＲＩ后２４ｈ、７ｄ视网膜各层厚度，同时计数视网膜神经节细胞数。结果　ＲＩＲＩ后２４ｈ及７ｄ，模型组视网膜电流图的ｂ波振幅分别为（９２．３３±２４．３６）μＶ及（１０１．２８±２１．９７）μＶ，较正常对照组大鼠测量到的（２１５．４０±３５．９１）μＶ显著降低，而治疗组的ｂ波振幅分别为（１３７．０７±２２７１）μＶ及（１６６．２４±１６．７２）μＶ，较模型组显著好转（均为Ｐ＜０．０５）；各组ａ波与ｂ波的变化趋势相同。大鼠ＲＩＲＩ后２４ｈ，模型组全层视网膜高度水肿，可见大量空泡变性，ＲＩＲＩ后７ｄ，视网膜厚度明显变薄，而治疗组大鼠视网膜的组织病理学损害较模型组均明显减轻。结论　复方丹参滴丸可从形态学及功能学上减轻大鼠ＲＩＲＩ损伤。

Protective effects of compound danshen dripping pills on retinal ischemia-reperfusion injury in Rats

Objective To study the effects of compound danshen dripping pills on retinal ischemia-reperfusion injury ( RIRI) in rats. Methods Forty-two adult healthy SD rats were randomly divided int0 3 groups : Sham group( 6 rats) ,model group (18 rats) and treatment group ( 450 mg . kg -l . d-’) ( 18 rats). The model of RIRI was established by increasing the intraocular pressure via cannulation into the anterior chamber. Electroretinogram( ERG) was recorded at 24 hours and 7 days after RIRI. respectively , then pathological changes of the retina were observed with light microscope. Thicknesses of the retinal layers and RGC cell counts were calculated at 24 hours and 7 days respectively after RIRI. Results At 24 hours and 7 days after RIRI, the b-wave amplitudes of the model group were ( 92. 33 + 24. 36) VV and ( 101. 28 +21. 97 ) VV, respectively , which were much lower than the sham group ( 215. 40 + 35. 91 ) VV. While the b-wave amplitudes of the treatment group were ( 137. 07 + 22. 71) VV and ( 166. 24 + 16. 72) yV,respectively , which were higher than the model group ( all P < 0. 05 ) . In the model group , all layers of the retinas were highly edema with enormous vacuolar degeneration at 24 hours after RIRI, and the thicknesses of retinas were greatly reduced at 7 days after RIRI. as shown in the HE analysis. These insults were reversed to some extent in the compound danshen dripping pills treatment group. Conclusion Compound danshen dripping pills may have a protective effect on the retinal morphology as well as the retinal function after RIRI.