| Arthrobacter globiformis酯酶基因的克隆、表达和酶活性测定 |
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| 引用本文： | 李新月,叶蕊芳,郑黎,郑一涛,李晴,薛建萍. Arthrobacter globiformis酯酶基因的克隆、表达和酶活性测定[J]. 医学教育探索, 2006, 0(8): 930-932979 |
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| 作者姓名： | 李新月 叶蕊芳 郑黎 郑一涛 李晴 薛建萍 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室 上海200237(李新月，叶蕊芳，郑黎，郑一涛，李晴)，上海农药研究所 上海200032(薛建萍) |
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| 摘 要： | 采用聚合酶链反应(PCR)的方法扩增了A rthrobacter g lobiform is中酯酶编码基因,与载体质粒连接后在大肠杆菌BL 21(DE 3)中进行表达。以包涵体形式表达的蛋白在8 m o l/L尿素作用下溶解,经过透析复性后获得了具有活性的粗蛋白。产物活性实验表明,酯酶表现出较高的催化活性,为菊酯作为杀虫剂的应用奠定了基础。
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| 关 键 词： | 酯酶基因 克隆 表达 活性测定 |
| 收稿时间： | 2005-08-29 |
Cloning and Expression of the Esterase Gene of Arthrobacter globiformis and the Activity Assay of the Esterase |
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| LI Xin-yue,YE Rui-fang,ZHENG Li,ZHENG Yi-tao,LI Qing,XUE Jian-ping. Cloning and Expression of the Esterase Gene of Arthrobacter globiformis and the Activity Assay of the Esterase[J]. Researches in Medical Education, 2006, 0(8): 930-932979 |
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| Authors: | LI Xin-yue YE Rui-fang ZHENG Li ZHENG Yi-tao LI Qing XUE Jian-ping |
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| Abstract: | A novel esterase which stereoselectively hydrolyzes the( )-trans stereoisomer of ethyl chrysanthemate to produce( )-trans-chrysanthemic acid was discussed.The gene coding for the esterase was amplified from Arthrobacter globiformis by polymerase chain reaction(PCR).The gene was inserted into a vector and expressed in Escherichia coli BL21(DE3).The protein expressed in body was dissolve in 8 mol/L urea before dialysis.The purified crude enzyme show high catalysis activity.It may suggest the possibility that( )-trans-chrysanthemate is identified as the insecticide. |
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| Keywords: | esterase gene clone expression esterase activity |
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