HPLC法测定双黄连注射液中木犀草苷的含量

目的:建立高效液相色谱法同时测定双黄连注射液中木犀草苷含量的方法.方法:采用高效液相色谱法.色谱柱为Agilent ZORBAX SB - phenyi(4.6mm×250mm,5μm),流动相为乙腈(A)-0.5％冰醋酸(B)(A+B=100％)作梯度洗脱,流速:0.8ml*min -1,检测波长为350nm,柱温为室温,供试品进样量30μL、对照品进样量10μL.结果:木犀草苷的进样量在0.82～4.91μg (r=0.9999)范围内与各自峰面积积分值呈良好的线性关系；平均回收率为103.31％,RSD为1.87％(n=6).结论:表明该方法简单、快速、准确,可用于双黄连注射液的质量控制.

Determination of luteoHn in Shuanghuanglian injection by HPLC

OBJECTIVE： To establish a HPLC method for the content determination of luteolin in Shuanghuanglian injection. METHODS： HPLC method was adopted. Agilent ZORBAX SB -phenyl （4. 6mm × 250ram, 5 μm ） column was used. Acetonitrile - 0. 5% acetic acid was used as mobile phase at the flow rate of 0. 8 ml ＊ min-l , and the detection wavelength was set at 350 nm. colum temperature for room temperature, for try products into sample volume 30μL, and controlled products into sample volume 10μL RE- SULTS： The linear ranges of luteolin was 0. 82 -4. 91μg （R =0. 9999） respectively. The average recoverie was 103.31% , RSD = 1.87% , （n = 6）, respectively. CONCLUSION： The method is simple, rapid and accurate, which can be used for quality control of Shuanghuanglian injection.