Conservation of HPV-16 E6/E7 ORF sequences in a cervical carcinoma. |
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| Authors: | R W Cone A C Minson M R Smith J K McDougall |
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| Institution: | First Hill Women's Clinic, Seattle, Washington. |
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| Abstract: | A cervical carcinoma that contained human papillomavirus (HPV)-16 homologous DNA was analyzed. Each tumor cell genome contained a single, incomplete copy of HPV-16 DNA. The E6 and E7 open reading frames (ORFs) were completely conserved relative to other published HPV-16 sequences. Much of the non-coding region (NCR) was free of base changes, including complete conservation of several regulatory elements. Multiple mutations were identified in the remaining integrated HPV-16 DNA, which was composed of parts of the L1 and E1 ORFs. The extraordinary conservation of the E6/E7 DNA sequence, as compared with other regions of the integrated HPV-16 DNA, supports the role of E6/E7 in tumorigenesis. |
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| Keywords: | human papillomavirus genital cancer |
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