先天性膈疝大鼠胎肺体外培养模型的建立

目的：建立先天性膈疝大鼠胎肺体外培养模型。方法：孕鼠Nitrofen灌胃，图像分析方法统计先天性膈疝大鼠胎肺体外培养模型的肺胚芽的发育参数（面积和周长），统计数值以x^-±s表示，并对结果进行t检验。结果：对照组与肺胚芽发育明显比Nitrofen组成熟，培养96h后，可见胚芽发育并增殖；Nitrofen组肺胚芽发育差，培养72h后肺胚芽逐渐出现发育停止和坏死的情况；96h后，两组肺发育参数肺面积[（2．535±0．423）mm^2 vs（1．053±0．033）mm^2]及周长[（6．544±0．710）mm vs（4．862±0．458）mm]相比均有统计学意义（P〈0．05）。结论：Nitrofen在胚胎发育早期导致肺发育不良，适于先天性膈疝大鼠胎肺体外培养模型建立。

Establishing the Culture Model of Congenital Diaphragmatic Hernia

Objective：To introduce how to establish the culture model of congenital diaphragmatic hernia. Methods： Image analysis the development parameter（ area and perimeter） in nitrofen-induced hypoplastic foetal rat lung explants, ± s show statistics and t test. Results：In control group,the development of the lung explants was mature and lung buds grew flourishing after 96 h of culture. Conversely, the Nitrofen group took place developmental necrotic and cellular necrosis gradually after 72 h. Lung area [ （ 2. 535 ±0. 423 ） mm^2 vs （ 1. 053 ±0. 033 ） mm^2 ] and perimeter [ （6. 544 ±0. 710 ） mm vs （4. 862 ±0. 458 ） mm ] were significant differences in control group compared with Nitrofen after 96 h of culture （ respectively ; P 〈 0.05 ）. Conclusion ： Nitrofen lead to pulmonary hypoplasia in earlier period of embryonic development.