猫脑弥漫性轴索损伤扫描电镜观察

目的:探讨弥漫性轴索损伤(Diffuse axonal injury,DAI)的法医学诊断依据。方法:采用头颅半约束非撞击性旋转暴力致伤方法建立猫脑弥漫性轴索损伤模型。在致伤后不同时间对脑组织进行了HE染色、Bielshowsky Glee银染色和扫描电镜观察。结果:HE染色显示2米损伤组及3米损伤组均未见特征性的改变;Bielshowsky Glee银染色显示2米损伤组未见特征性改变,3米损伤组伤后6小时见收缩球形成,伤后12小时达到高峰,持续至24小时;扫描电镜显示2米损伤组未见特征性改变,3米损伤组伤后4小时可检见收缩球形成,随着时间延长其体积逐渐增大,数量逐渐增多,于12小时达到高峰,持续至24小时。结论:扫描电镜对弥漫性轴索损伤的诊断价值优于HE染色、Bielshowsky Glee银染色。扫描电镜形态学改变对弥漫性轴索损伤的诊断及损伤时间推断有一定的参考价值。

The SEM Observation of Diffuse Axonal Injury of Brain in Cat

Objective:To disclose the indicators for identifying diffuse axonal injury in forensic practice.Methods Establish a animal model of diffuse axonal injury(DAI)caused by nonimpact injury with half bound head in cats.the cerebral sample stained by H.E,Bielshowsky Glee and observed by Scanning Electron Microscope at different intervals after injury.Results H.E staining:there were no distinctive change in 2 and 3 meter in height injury groups;Bielshowsky Glee staining:there were no distinctive change in 2 meters group,in 3 meters group,retraction balls have been observed at 6 hours after brain injury and reach the peak at 12 hour and keep on till 24 hours.Scanning Electron Microscope observation:there were no distinctive change at 2 meters group,retraction balls have been observed at 4 hours after brain injury in the 3 meters group,it's bulk and quantity have gradually increasing while the dating of injury has been prolonged and it reach the peak at 12 hour and keep on till 24 hours.Conclusions To diagnose diffuse axonal injury by Scanning Electron Microscope observation is better than H.E staining and Bielshowsky Glee staining.The morphological change observed by Scanning Electron Microscope have some 1value in dating of wound of diffuse axonal injury.