外源核苷酸对小鼠免疫功能的影响

目的 :　本实验研究了日粮核苷酸促进免疫抑制小鼠的免疫功能恢复的作用。方法 :　选取 5～ 6 w龄 ,体重为 (2 0± 2 ) g的健康昆明种小鼠 6 0只 ,设 0、0 .0 5 %、0 .2 5 %三个核苷酸水平处理 ,每个处理中又分成正常和免疫抑制两个组 ,其中所有免疫抑制组在实验的第 2 d一次性大剂量腹腔注射环磷酰胺 (1 5 0 mg/kg bw) ,制备免疫抑制模型 ;正常组注射等同剂量的生理盐水。饲养 1 0 d后观察小鼠增重、脾脏和胸腺的脏器指数、脾淋巴细胞转化率、脾脏中抗 SRBC抗体形成细胞数量以及血清中抗 SRBC抗体水平。结果 :　日粮中添加核苷酸能够提高正常小鼠的增重 (P<0 .0 5 )、胸腺指数、淋巴细胞转化率 (P<0 .0 5 )及血清中抗体水平 ,极显著提高免疫抑制小鼠抗体水平 (P<0 .0 1 ) ,使抑制小鼠的各项指标接近正常。抗 SRBC抗体形成细胞数量没有显著变化。结论 :　在无核苷酸日粮中添加核苷酸能够促进正常小鼠免疫功能的提高 ,使免疫抑制小鼠免疫功能得到改善。

EFFECTS OF EXOGENOUS NUCLEOTIDES ON IMMUNE FUNCTION IN MICE

Objective: To study the effects of exogenous nucleotides on immune function in mice.Methods: Sixty Kunming mice, (20±2) g, 5-6 weeks old were divided into three treatments groups (fed 0,0.05%,0.25% nucleotide-supplemented diet respectively). Each treatment was divided into two groups: normal group and immunodepression group. Mice in immunodepression groups were injected with cyclophosphamide (150 mg/kg bw) on the 2nd day in order to suppress the immune function and the mice in normal group were treated with normal saline at the same time. All mice were injected with SRBC on the 6th day. 0,0.05%,0.25% nucleotides were added into basic diet respectively and fed for 10 days. Body weight gain, organ index (thymus and spleen), SI, antibody anti SRBC and antibody secreting cell number in spleen were measured. Results: The body weight gain(P<0.05), SI (P<0.05)of normal rats and antibody (P<0.01)of immunodepression mice were increased significantly with nucleotides supplement.Conclusion: Exogenous nucleotides can accelerate the recovery of immune function of immunodepression mice and improve the immune function of normal mice.