氟对雄性大鼠生殖系统的内分泌干扰作用研究

目的　探讨高氟对雄性大鼠生殖系统的内分泌干扰作用。方法　给予雄性Wister大鼠含氟化钠 15 0mg/l的水 10周后 ,检测其精子计数、活动率、畸形率 ,测定睾丸、附睾生化标志酶和性激素 ,观察睾丸组织病理改变。结果　染毒组精子计数、活动率下降 ,畸形率升高 ,血清睾酮、促黄体生成激素下降 ,较对照组差异有统计学意义 (P <0 .0 5 ) ,睾丸组织有明显的病理学改变。结论　长期接触高浓度的氟对雄性生殖系统有明显的内分泌干扰作用

Experimental study on endocrine disruption effect of fluoride on reproductive system of male rats

Aim:To study the endocrine disruption effects of excessive fluorine on reproductive system of male rats.Methods:Sodium Fluoride was administered to male rats with drinking water for 10 weeks.Then examine the sperm count,the rate of sperm mobility、aberration,the level of Lactate dehydrogenase(LDH)、Na +K +-ATPase、Mg 2+ -ATPase、Ca 2+ -ATPase and serum Testosterone(T)、Luteinizing hormone(LH),and observe the pathological alterations of testes.Result:The sperm count,the rate of sperm mobility and the level of LDH,Na +K +-ATP、Mg 2+ -ATP、Ca 2+ -ATP and serum T、LH of the fluoride group were lower,the rate of aberration was upper.The pathological alterations of testes were observed.The differences between two groups were significant(P<0.05).Conclusions:The long-term exposure to fluorine of high dose may produce endocrine disruption effect on male reproductive system.