新型生物创伤敷料的制备与研究

目的 以猪小肠黏膜下层为原料制备新型生物敷料.方法 按Abraham方法制备猪小肠黏膜下层,冻干后冷冻粉碎为直径15～30 μm的小肠黏膜下层粉末,将该粉末置于含有1%胃蛋白酶和3%乙酸的水溶液中混合消化48h,将得到的小肠黏膜下层溶液冷冻干燥制备成小肠黏膜下层海绵,最后采用碳化亚胺对干燥后的海绵进行交联改性,从而得到猪小肠黏膜下层海绵.通过形态结构、吸水率、降解性能等方面的研究,对其作为创伤敷料的有效性进行初步评价.结果 小肠黏膜下层海绵呈三维网孔状结构,孔径为100～200μm.1%SIS海绵具有很强的吸水能力并在磷酸缓冲液中具有较好的稳定性,1%SIS海绵在大鼠骨骼肌内埋置4w后可完全降解,周围骨骼肌纤维修复良好,无变性、坏死等不良现象发生.结论 猪SIS海绵具备作为新型生物敷料的潜能,具有止血、防渗出能力强的特点,能为细胞的生长和繁殖提供营养和代谢环境,可完全降解并达到组织永久性替代的目的.

Preparation and investigation of a novel wound dressing

Objective To prepare and investigate porcine small intestine submucosa sponges as a novel wound dressing. Method The porcine small intestine submucosa （SIS） was prepared according to the method described by Abraham et al. The SIS was freeze-dried using freezer dryer. The dried SIS was pulverized using freezer mill to give approximate 15-30μm size of SIS powder.The obtained SIS powders were added in aqueous solution consisting of 3% acetic acid and 0.1%pepsin, and then dissolved for 48 h. SIS solution was poured into polystyrene petri dishesto give SIS sponge shape, followed by freeze-drying. SIS sponges were crosslinked with 50 mM N-（3-dimethylaminopropyl）-N＇-ethylcarbodiimide hydrochloride （EDC） for 24 h. The tests including of morphological observation, water absorption ability and biodegradation were done to valuate the validity of SIS sponges as wound dressing. Result SEM showed that SIS sponge had interconnected network structure and regular pore. The pore diameter was around 100-200 μm.The SIS sponges prepared by 1% SIS concentration exhibited high water absorption ability and low weight loss in PBS. In vivo implantation test, the sponges were implanted in dorsal muscle of rats for 4 weeks and the results suggest that the SIS sponges had good biocompatibility and also could completely degrade in vivo. Conclusion The SIS sponges had potential biological properties as a novel wound dressing.