枸杞多糖对大鼠海马缺血再灌注损伤的干预作用

目的:观察枸杞多糖(lycium barbarum polysaccharide,LBP)对大鼠海马缺血再灌注损伤后超氧化物歧化酶(superoxide dismutase,SOD)的活性和白介素6(interleukin 6,IL-6)含量的影响。方法:选用健康成年SD大鼠,雌雄不拘,随机分成3组:对照组、缺血再灌注组和枸杞多糖组。脑缺血模型采用线栓法致大脑中动脉阻闭,2h后抽出栓塞线,再灌注24h后处死大鼠,测定海马SOD活性和IL-6的含量。结果:(1)枸杞多糖对缺血再灌注大鼠海马SOD活性的影响:缺血再灌注组大鼠海马SOD活性显著低于对照组(P<0.01),枸杞多糖组大鼠海马SOD活性低于对照组,但显著高于缺血再灌注组(P<0.01)。(2)枸杞多糖对缺血再灌注大鼠海马IL-6的影响:枸杞多糖组大鼠海马IL-6含量与正常对照组相比差异没有统计学意义,而与缺血再灌注组大鼠相比明显下降(P<0.05)。结论:枸杞多糖可提高缺血再灌注大鼠海马SOD活性,降低IL-6的含量,对缺血再灌注损伤有保护作用。

Influence of Lycium Barbarum Polysaccharide on Hippocampus in Rats with Ischemia-perfusion Injury

Objective：To observe the effect of lycium barbarum polysaccharide（LBP） on superoxide dismutase（SOD） and interleukin 6（IL-6） in hippocampus of rats with ischemia-perfusion injury.Methods：Healthy adult SD rats were divided into three groups randomly： normal control group（C）,ischemia-perfusion group（IR）and lycium barbarum polysaccharide group（LBP）.Cerebral ischemia-perfusion model of rat was made by middle cerebral artery occlusion（MACO） with 3-0 nylon monofilament for 120 minutes.The activity of SOD and content of IL-6 in hippocampus of rats were detected after 24 hours reperfusion. Results： （1）Influence of LBP on the activity of SOD.. activity of SOD in IR group was significantly lower than that of C group （P（0. 01）. The activity of SOD in LBP group was lower than that of C group,while higher than that of IR group significantly（P（0. 01）. （2）Influence of LBP on the content of IL-6： the content of IL-6 had no significant difference compared to that of C group and was lower than that of IR group（P（ 0.05）. Conclusion：The study indicated that LBP could improve the activity of SOD and decrease the content of IL-6. LBP may relate with protection of ischemia-perfusion injury.