通过小鼠动情期的确定制作含有早胚石蜡切片 |
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引用本文: | 苏秋荧,雷玉芳,张小凤,欧阳福,任亚萍.通过小鼠动情期的确定制作含有早胚石蜡切片[J].华夏医学,2014,27(4):47-49. |
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作者姓名: | 苏秋荧 雷玉芳 张小凤 欧阳福 任亚萍 |
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作者单位: | 苏秋荧 (桂林医学院,广西桂林,541004); 雷玉芳 (桂林医学院,广西桂林,541004); 张小凤 (桂林医学院,广西桂林,541004); 欧阳福 (桂林医学院,广西桂林,541004); 任亚萍 (桂林医学院组织胚胎学教研室,广西桂林,541004); |
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基金项目: | 2012年国家级大学生创课题资助(项目编号:201210601003)2012年广西区大学生创新课题资助(项目编号:2012593)2011年桂林医学院项目驱动课题资助(项目编号:2011005) |
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摘 要: | 目的:采用石蜡切片技术制作早期胚胎切片模型。方法:根据阴道脱落细胞检测法确定动情期最佳受孕时间,通过体内受精方法获得小鼠早期胚胎,采用石蜡切片技术制作早期胚胎切片模型。结果:将受孕小鼠断颈处死取各期胚胎,制作含有早胚石蜡切片。结论:本实验组为进一步对早期胚胎发育的研究提供了技术基础。
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关 键 词: | 昆明小鼠 动情期 胚胎 石蜡切片 |
Production of paraffin section containing early embryo through the determination of estrus in mice~[ |
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Institution: | SU Qiu-ying , LEI Yu-fanga, ZHANG Xiao-fenga, et al( Undergraduate of 2010, Clinical Medicine of Guilin Medical University, Guilin 541004, China.) |
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Abstract: | Objective: To adopt paraffin section technique to obtain the section model of early embryo. Methods: The most favorable fertilization time was confirmed in estrus period by testing vaginal ex foliated cells; the early mice embryo was obtained by internal fertilization, and the early embryonic section model was obtained through paraffin section technique. Results: The pregnant mice was broken the neck to death, embryos of various periods were taken, and the paraffin section containing the early embryos could be made. Results: The experimental team have provided technology basis for further research of the early embryonic development. |
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Keywords: | Kunming mice estrus period embryo paraffin section |
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