中药清毒液对急性肺损伤兔肺组织和血浆一氧化氮和内皮素水平的影响

目的:探讨中药清毒液(QD)对急性肺损伤(ALI)肺组织及血浆一氧化氮(NO)和内皮素1(ET1)水平的影响及其对肺损伤的防治作用。方法:采用间隔24h两次注射大肠杆菌内毒素(LPS)的方法,复制家兔内毒素急性肺损伤模型。结果:清毒液能明显抑制内毒素所引起的家兔血浆、肺组织及支气管肺泡灌洗液(BALF)中NO和ET1水平升高(P<0.01),BALF内中性粒细胞(PMN)增多,肺水含量及通透指数升高,并改善病理变化。结论:中药清毒液可阻抑内毒素对家兔肺组织的损伤作用。

Effects on the Levels of Nitric Oxide and Endothelin-l of Lung Tissue and Plasma in Rabbits with Acute Lung Injury Using the Decoction of Traditional Chinese Medicines of Antipyretic and Relieving Internal Fever.

Objective:To explore the effects on the levels of nitric oxide(NO) and endothelin-1(ET-1) and its potential functions in rabbits acute lung injury(ALI) using the decoction of traditional Chinese medicines of antipyretic and relieving internal fever.Methods:The ALI model was induced by injecting E.Coli endotoxin at twice with an interval of 24 hours.Results:NO and ET-1 levels in plasma, lung tissue and bronchoalveolar lavage fluid(BALF) increased significantly(P<(0.01)) in ALI group.WBC in BALF, lung index,and lung permeability index(LPI) also increased significantly(P<0.01).In ALI group, the levels plasma NO and ET-1, but their temporal dynamics were different. The increase of plasma NO and IL-8 concentration reached its peak at 120 min after endotoxin injection. While plasma ET-1 concentration increased much earlier at 60 min post-endotoxin and its plasma concentration had reached its peak level, then waned, but restored that level approach to normal rabbit at 6 hours post-injury in lung.Conclusion:NO and ET-1 might be an important liquit factor in different phases of ALI.All NO and ET-1 maybe involve in pathogenesis of ALI.