| 钉螺转氨酶的初步研究 |
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| 引用本文： | 王根法,宋庚明.钉螺转氨酶的初步研究[J].中国寄生虫学与寄生虫病杂志,1990,8(2):110-112. |
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| 作者姓名： | 王根法 宋庚明 |
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| 作者单位： | 中国预防医学科学院寄生虫病研究所 世界卫生组织疟疾、血吸虫病及丝虫病合作中心
(王根法)，中国预防医学科学院寄生虫病研究所 世界卫生组织疟疾、血吸虫病及丝虫病合作中心(宋庚明) |
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| 摘 要： | 纸层析法证明丙氨酸、天门冬氨酸及精氨酸经过钉螺匀浆作用后可使α-酮戊二酸转变成谷氨酸,说明钉螺存在谷-丙、谷-草及谷-精转氨酶。比色法也测得谷-草转氨酶和谷-丙转氨酶的活力。溴乙酰胺对上述两酶活力无明显影响,而烟酰苯胺有抑制钉螺谷-丙转氨酶作用。小剂量溴乙酰胺与烟酰苯胺盐酸盐伍用,无增强杀螺作用。
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| 关 键 词： | 溴乙酰胺 钉螺 转氨酶 烟酰苯胺 |
PRELIMINARY STUDIES ON TRANSAMINASE OF ONCOMELANIA SNAIL |
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| Wang Gen-Fa,Song Geng-Ming Institute of Parasitic Diseases,Chinese Academy of Preventive Medicine.PRELIMINARY STUDIES ON TRANSAMINASE OF ONCOMELANIA SNAIL[J].Chinese Journal of Parasitology and Parasitic Diseases,1990,8(2):110-112. |
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| Authors: | Wang Gen-Fa Song Geng-Ming Institute of Parasitic Diseases Chinese Academy of Preventive Medicine |
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| Institution: | Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine. |
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| Abstract: | By paper chromatography, the tissue homogenate of Oncomelania snails was shown to form glutamic acid at the expense of alpha-ketoglutarate plus aspartic acid, alanine or arginine respectively. The existence of alanine-glutamate, aspartate-glutamate and arginine-glutamate transaminase in Oncomelania snail was demonstrated. By using colorimetric method, the activity of aspartate-glutamate transaminase (GOT) and alanine-glutamate transaminase (GPT) of Oncomelania snail was 1.64 +/- 0.01 and 0.99 +/- 0.01 mumol/h.mg protein respectively. GOT and GPT were not inhibited by 2 ppm bromoacetamide, but the activity of GPT was suppressed (40%) by 2 ppm nicotinanilide. A combination of 0.5 ppm bromoacetamide and 0.5 ppm nicotinanilide had no synergitic molluscicidal effect. |
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| Keywords: | Bromoacetamide nicotinanilide Oncomelania snail transaminase GOT GPT |
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