沙利度胺对子宫内膜异位症的内膜间质细胞体外表达VEGF的影响

目的:观察沙利度胺(ThD)对子宫内膜异位症病人内膜间质细胞的增殖及其血管内皮生长因子(VEGF)表达的影响。方法:刮取子宫内膜异位症病人的子宫内膜组织,经体外分离、培养,用波形蛋白单抗进行免疫组化染色鉴定,应用四唑盐(MTS)分析肿瘤坏死因子α(TNF-α)和ThD对间质细胞生长的影响,以实时荧光定量PCR、Western Blot和定量ELISA分析在存在外源TNF-α的情况下,不同浓度ThD对间质细胞表达VEGF的影响。结果:TNF-α能促进子宫内膜间质细胞的增殖和分泌VEGF,而ThD能剂量依赖性抑制内膜间质细胞的增殖,并逆转TNF-α对VEGF的上调作用。结论:ThD可抑制体外培养的子宫内膜异位症内膜间质细胞的增殖及其VEGF的表达和分泌,提示ThD对于治疗子宫内膜异位症可能具有一定的作用。

Effects of thalidomide on vascular endothelial growth factors expression in endometrial stromal cells of endometriosis in vitro

AIM:To observe the effects of thalidomide (ThD) on cell proliferation and vascular endothe- lial growth factors (VEGF) expression in endometrial stromal cell of endometriosis in vitro.METHODS:Human endometrial tissue was obtained from patients with endometriosis.The stromal celXs were isolated and identified by immunohistochemical staining with vimentin antibody.The influence of tumor necrosis factor-alpha (TNF-α) and ThD on proliferation of endometrial stromal cells was observed.The effects of different concentration of ThD on VEGF expression in endometrial stromal cells were analyzed by real-time PCR,Western Blot and ELISA. RESULTS:TNF-αcould promote the proliferation of endometrial stromal cell and the secretion of VEGF,while ThD could inhibit the proliferation of endometrial stromal cell and reverse the up-regulation of VEGF expression by TNF-α.CONCLUSION:ThD could inhibit the proliferation of endometrial stromal cell and expression and secretion of VEGF in vitro,which suggested that ThD might have therapeutic effect on endometriosis.