血液透析患者外周血单核细胞COX-2的表达及其与炎症细胞因子水平的关系

目的探讨血液透析患者外周血单核细胞环氧合酶-2（COX-2）的表达与炎症细胞因子IL-6、IL-8水平的关系。方法采用ELISA的方法测定48例维持性血液透析患者和25例健康对照组外周血单核细胞培养上清中COX-2、IL-6、IL-8水平,并测定两组观察对象血清高敏C反应蛋白（hs-CRP）的浓度。结果血液透析患者hs-CRP、COX-2、IL-6、IL-8水平明显高于健康对照组,COX-2与IL-6、IL-8、hs-CRP之间存在相关性。结论维持性血液透析患者存在微炎症状态,COX-2与IL-6、IL-8之间可能存在的正反馈效应促进患者微炎症状态的发展。

COX-2 expression in mononuclear cells of peripheral blood and the relationship between COX-2 and inflammatory cytokines in hemodialysis patients

Objective To evaluate cyclooxyenase-2（COX-2） expression in mononuclear cells of peripheral blood and the relationship between COX-2 expression and levels of inflammatory cytokines IL-6 and IL-8 in hemodialysis patients Methods We recruited 48 maintenance hemodialysis patients and 25 healthy volunteers in this study.ELISA was used to detect COX-2,IL-6 and IL-8 in the medium of cultured mononuclear cells from peripheral blood.Serum high sensitive C reactive protein（hs-CRP） was also measured.Results hs-CRP,COX-2,IL-6 and IL-8 levels were significantly higher in maintenance hemodialysis patients than in healthy controls.Corre-lation was found between COX-2 expression and levels of IL-6,IL-8 and hs-CRP.Conclusion Microinflammatory state presented in maintenance hemodialysis patients.Positive feedback between COX-2 expression and the levels of IL-6 and IL-8 may exist to promote the microinflammatory state in these patients.