基质金属蛋白酶-9和表皮生长因子受体在非小细胞肺癌中的表达及临床意义

目的 探讨基质金属蛋白酶-9（MMP-9）和表皮生长因子受体（EGFR）在非小细胞肺癌（NSCLC）中的表达及与临床、病理的关系。方法 对经随诊资料完整的术后非小细胞肺癌患者组织标本60例、癌旁组织加例和肺正常组织5例，采用S—P免疫组化法检测MMP-9和EGFR。结果 60例NSCLC中MMP-9蛋白的阳性表达率为63．33％，20例癌旁组织的阳性表达率为10％，5例正常组织未见阳性染色细胞。EGFR的阳性表达率为65．00％，20例癌旁组织及5例正常组织未见阳性染色细胞。癌组织MMP-9和EGFR与癌旁组织相比差异有显著意义（P〈0．05）。MMP-9的表达与肿瘤的TNM分期、淋巴结转移呈正相关（P〈0．05），EGFR与肿瘤大小、TNM分期、病理分级、淋巴结转移有显著性差异（P〈0．05）。MMP-9和EGFR的表达与患者的年龄、性别、吸烟习惯及肿瘤的病理类型间无显著性差异（P〉0．05）。结论 MMP-9和EGFR的信号传导对于非小细胞肺癌的侵袭和转移起着重要作用，MMP-9和EGFR的共同高表达是肺癌不良预后的指标。

Expression and clinical significance of MMP-9 and EGFR in patients with non-small cell lung cancer

Objective To investigate the expression of MMP-9 and EGFR in non-small cell lung cancer and they're relationship between them and the prognostic significance.Methods The expression of MMP-9 and EGFR were detected in 60 NSCLC samples,20 lung tissues adjacent to cancer and 5 normal lung tissues as control by immunohistochemical technique (streptavidin-peroxidase conjugate method,S-P).Results The positive expression of MMP-9 and EGFR in 60 cases NSCLC was 63.33% and 65.00% respectively which was significantly higher than that in 20 lung tissues adjacent to cancer and 5 normal lung tissues(P<0.05).The MMP-9 and EGFR positive expression were not correlated to age,sex,smoking histology and the pathologic types of tumor cell(P>0.05),The positive expression of MMP-9 protein was related with TNM staging and also related to lymph node metastasis(P<0.05),The positive expression of EGFR protein was related with tumor magnitude,cell differentiation and also related to TNM staging,lymph node metastasis(P<0.05).Conclusion MMP-9 and EGFR may play important roles in NSCLC invasion and metastasis.Over expression of MMP-9 and EGFR could act as a reference to evaluate metastasis and unfavorable prognosis of NSCLC.