| 接种IL—6基因转导的白血病细胞后局部病理形态学实验研究 |
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| 引用本文： | 葛林阜,曹雪涛.接种IL—6基因转导的白血病细胞后局部病理形态学实验研究[J].中华物理医学杂志,1996,18(3):156-158. |
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| 作者姓名： | 葛林阜 曹雪涛 |
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| 作者单位： | 济南军区总医院(葛林阜!250031，李劲松!250031，丁吉元!250031，王淑霞!250031)，上海第二军医大学(曹雪涛，于益芝)，空军济南医院(王云凤) |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 将人白细胞介素6(IL-6)基因转导的高分泌IL-6的FBL-3红白血病细胞克隆株体外扩增后,接种至C57BL/6小鼠皮下,观察肿瘤生长情况,并于不同时间取接种处组织作常规病理、免疫组化及电镜检查。结果表明接受IL-6基因转导的FBL-3细胞接种后的小鼠成瘤晚、生长速度慢,且肿瘤接种局部可见大量粒细胞和部分浆细胞、淋巴细胞的浸润,接种处组织免疫组化显示增殖细胞核抗原阳性率显著降低,提示IL-6基因转导的白血病细胞在体内致瘤性降低与其接种局部某些杀伤细胞浸润而诱导机体抗肿瘤免疫功能增强有关。
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| 关 键 词： | 白细胞介素6 基因转导 白血病细胞 病理学 免疫组化 超微结构 |
Pathological Features after Inoculated with IL-6 Gene-Transfected FBL-3 Cells |
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| Ge Linfu,Cao Xuetao,LiJinsong,et al..Pathological Features after Inoculated with IL-6 Gene-Transfected FBL-3 Cells[J].Chinese Journal of Physical Medicine and Rehabilitation,1996,18(3):156-158. |
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| Authors: | Ge Linfu Cao Xuetao LiJinsong |
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| Institution: | Ge Linfu,Cao Xuetao,LiJinsong,et al. Department of Hematology,General Hospital of Jinan Command PLA,250031 |
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| Abstract: | FBL-3 clone transfected with IL-6 gene Was estabished After expansion in vivo, the cione was inoculated into C57BL/6 mice subcutaneously for observation of fumor growth. Samples were taken from the site of inoculation at different time for pathological inspection and ultrastructure analysis. In mice received IL-6 gene transfected FBL-3 cells, the tumor growth rate was low with decreased positive rate of proliferative cellullar nuclear antigen and a massive infiltration of granuloicytes with a few plasma cells and lymphocytes around the tumor mass was obserived. The results demonstrated that enhanced immune response is related to the decreased tumorigenicity of FBL-3 cells transfected with IL-6 gene. |
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| Keywords: | interleukin-6 gene transfer 1eukemia cel1s pathology immunohistochemistry u1trastructure |
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